Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans

Yon Ju Ji, Jiou Wang

Research output: Contribution to journalArticle

Abstract

Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans is one of the best model systems to study heterochronic genes, since the subtle changes in the development of cell lineages can be easily identified. Among the different cell types in C. elegans, hypodermal seam cells and their lineages are well known to be maintained by lin-14, whose expression level is regulated by two miRNA genes, lin-4 and let-7, at the larval stages. Therefore, analyzing the heterochronic phenotype of hypodermal seam cells in C. elegans could yield detailed insights into the status of the miRNA pathway. Here we describe the assay protocol to analyze the heterochronic phenotypes of C. elegans hypodermal seam cells, which can be used as a reliable method to study the miRNA pathway.

Original languageEnglish (US)
JournalDevelopment (Cambridge, England)
Volume9
Issue number1
DOIs
StatePublished - Jan 5 2019

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Keywords

  • C. elegans
  • Heterochronic phenotype
  • Hypodermal seam cells
  • let-7
  • lin-14
  • lin-4
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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