Herpes simplex virus type II is not a cofactor to human papillomavirus in cancer of the uterine cervix

Danh Tran-Thanh, Diane Provencher, Anita Koushik, Eliane Duarte-Franco, Allégria Kessous, Pierre Drouin, Cosette M. Wheeler, Josée Dubuc-Lissoir, Philippe Gauthier, Guy Allaire, René Vauclair, Joseph A. DiPaolo, Patty Gravitt, Eduardo Franco, Francois Coutlée

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: Cells that were cotransfected with herpes simplex virus-16 and the herpes simplex virus type 2 Xho-2 DNA induce tumors in nude mice. In a cross-sectional study, we investigated the role of herpes simplex virus type 2 as a cofactor to human papillomavirus in cervical cancer. STUDY DESIGN: Cervical cells that were obtained with an endocervical Cytobrush brush (Medscand) from 439 women (50 women with cancer lesions, 65 women with high-grade squamous intraepithelial lesions, 80 women with low-grade squamous intraepithelial lesions, 244 healthy subjects) and DNA that was extracted from 150 cervical cancer biopsy specimens were analyzed with polymerase chain reaction for herpes simplex virus type 2 Xho-2 and Bgl IIC transforming DNA sequences. RESULTS: All 439 cervical samples and 150 cervical cancer biopsy specimens tested negative for herpes simplex virus type 2 Xho-2 and Bgl IIC DNA by polymerase chain reaction. Overall, none of 200 samples (0%) from women with invasive cervical cancer contained herpes simplex virus type 2 Xho-2 or Bgl IIC DNA (95% Cl, 0.0-1.8). CONCLUSION: Although herpes simplex virus type 2 BglIIN transforms epithelial cells in vitro, it was not detected in cervical cancer specimens.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume188
Issue number1
DOIs
Publication statusPublished - Jan 1 2003
Externally publishedYes

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Keywords

  • Cervical cancer
  • Chlamydia trachomatis
  • Herpes simplex virus-2
  • Human papillomavirus

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Tran-Thanh, D., Provencher, D., Koushik, A., Duarte-Franco, E., Kessous, A., Drouin, P., ... Coutlée, F. (2003). Herpes simplex virus type II is not a cofactor to human papillomavirus in cancer of the uterine cervix. American Journal of Obstetrics and Gynecology, 188(1), 129-134. https://doi.org/10.1067/mob.2003.66