Herpes simplex virus 1 (HSV-1) DNA and immune complex (HSV-1-human IgG) elicit vigorous interleukin 6 release from infected corneal cells via Toll-like receptors

Kozaburo Hayashi, Laura C. Hooper, Marian S. Chin, Chandrasekharam N. Nagineni, Barbara Detrick, John J. Hooks

Research output: Contribution to journalArticle

Abstract

Toll-like receptor 3 (TLR-3) gene expression and interleukin 6 (IL-6) secretion were studied in corneal cells with herpes simplex virus (HSV). Human corneal epithelial cells (HCEs) and primary human corneal fibroblasts (HCRFs) were infected with live HSV or UV-inactivated HSV (UV-HSV), transfected with HSV DNA or treated with HSV-anti-HSV IgG immune complexes. Gene expression of TLR-3 and -9 was analysed by real-time PCR. Supernatants were assayed for IL-6 by ELISA. Incubation of HCEs and HCRFs with live HSV-1, UV-HSV and HSV DNA resulted in augmented TLR-3 and -9 gene expression and IL-6 release. Moreover, infected or transfected HCRFs released greater amounts of IL-6 than did HCEs. As virus is frequently in the form of neutralized virus immune complexes, the ability of these immune complexes to interact with TLRs and trigger IL-6 production was evaluated. Here, it is shown that HSV-anti-HSV IgG complexes were as potent as HSV DNA in their ability to induce IL-6. Treatment of HCRFs transfected with HSV DNA with the TLR-9-inhibitory oligomer iODN, anti-TLR-3 antibody or phosphatidylinositol 3-kinase inhibitor indicated that IL-6 release from HCRFs was mediated by TLR-3 and -9 gene expression. These results demonstrated that neutralized HSV immune complexes were as potent as HSV DNA in enhancing IL-6 release from corneal fibroblasts. These phenomena were mediated via augmented TLR-3 and -9 gene expression.

Original languageEnglish (US)
Pages (from-to)2161-2169
Number of pages9
JournalJournal of General Virology
Volume87
Issue number8
DOIs
StatePublished - Aug 2006

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Toll-Like Receptors
Human Herpesvirus 1
Simplexvirus
Antigen-Antibody Complex
Interleukin-6
Immunoglobulin G
Toll-Like Receptor 3
DNA
Toll-Like Receptor 9
Fibroblasts
Gene Expression
Epithelial Cells
Phosphatidylinositol 3-Kinase
Viruses
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Virology
  • Immunology

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Herpes simplex virus 1 (HSV-1) DNA and immune complex (HSV-1-human IgG) elicit vigorous interleukin 6 release from infected corneal cells via Toll-like receptors. / Hayashi, Kozaburo; Hooper, Laura C.; Chin, Marian S.; Nagineni, Chandrasekharam N.; Detrick, Barbara; Hooks, John J.

In: Journal of General Virology, Vol. 87, No. 8, 08.2006, p. 2161-2169.

Research output: Contribution to journalArticle

Hayashi, Kozaburo ; Hooper, Laura C. ; Chin, Marian S. ; Nagineni, Chandrasekharam N. ; Detrick, Barbara ; Hooks, John J. / Herpes simplex virus 1 (HSV-1) DNA and immune complex (HSV-1-human IgG) elicit vigorous interleukin 6 release from infected corneal cells via Toll-like receptors. In: Journal of General Virology. 2006 ; Vol. 87, No. 8. pp. 2161-2169.
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abstract = "Toll-like receptor 3 (TLR-3) gene expression and interleukin 6 (IL-6) secretion were studied in corneal cells with herpes simplex virus (HSV). Human corneal epithelial cells (HCEs) and primary human corneal fibroblasts (HCRFs) were infected with live HSV or UV-inactivated HSV (UV-HSV), transfected with HSV DNA or treated with HSV-anti-HSV IgG immune complexes. Gene expression of TLR-3 and -9 was analysed by real-time PCR. Supernatants were assayed for IL-6 by ELISA. Incubation of HCEs and HCRFs with live HSV-1, UV-HSV and HSV DNA resulted in augmented TLR-3 and -9 gene expression and IL-6 release. Moreover, infected or transfected HCRFs released greater amounts of IL-6 than did HCEs. As virus is frequently in the form of neutralized virus immune complexes, the ability of these immune complexes to interact with TLRs and trigger IL-6 production was evaluated. Here, it is shown that HSV-anti-HSV IgG complexes were as potent as HSV DNA in their ability to induce IL-6. Treatment of HCRFs transfected with HSV DNA with the TLR-9-inhibitory oligomer iODN, anti-TLR-3 antibody or phosphatidylinositol 3-kinase inhibitor indicated that IL-6 release from HCRFs was mediated by TLR-3 and -9 gene expression. These results demonstrated that neutralized HSV immune complexes were as potent as HSV DNA in enhancing IL-6 release from corneal fibroblasts. These phenomena were mediated via augmented TLR-3 and -9 gene expression.",
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