Hepatoma-derived growth factor is expressed after vascular injury in the rat and stimulates smooth muscle cell migration

Jill V. Narron, Tamara D. Stoops, Kurt Barringhaus, Martin Matsumura, Allen D. Everett

Research output: Contribution to journalArticlepeer-review

Abstract

The role of hepatoma-derived growth factor (HDGF), a novel nuclear-targeted vascular smooth muscle cell (SMC) mitogen in vascular injury is unknown. We hypothesized that HDGF plays a role in SMC proliferation and migration in formation of the neointima after balloon injury of the rat carotid. Using co-immunohistochemical staining, HDGF and proliferating cell nuclear antigen (PCNA) were co-expressed in 80% of nuclei of neointimal cells 7 d post carotid balloon injury with HDGF. The HDGF-positive medial and neointimal cells were smooth muscle actin negative and therefore likely represented a subgroup of SMC that have undergone phenotypic switching. Utilizing modified Boyden chamber migration assays, adenoviral-expressed HDGF in mouse SMC increased migration 10-fold (20 versus 2). HDGF gene silencing reduced both SMC proliferation and migration. In conclusion, HDGF is highly expressed in the media and neointima post balloon injury, a SMC mitogen and positive regulator of cell migration. We speculate that HDGF is involved in the SMC proliferative and migratory response to injury resulting in neointimal formation.

Original languageEnglish (US)
Pages (from-to)778-783
Number of pages6
JournalPediatric research
Volume59
Issue number6
DOIs
StatePublished - Jun 2006

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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