TY - JOUR
T1 - Hepatocyte growth factor enhances intestinal mucosal cell function and mass in vivo
AU - Kato, Yoshifumi
AU - Yu, Dahong
AU - Lukish, Jeff R.
AU - Schwartz, Marshall Z.
PY - 1997/7
Y1 - 1997/7
N2 - Hepatocyte growth factor (HGF), originally known to stimulate hepatocyte DNA synthesis, recently has been shown to enhance growth of intestinal epithelial cells in vitro. However, there have been no studies on the effect of HGF on the function of intestinal epithelial cells in vivo. This study was designed to examine the effect of systemically administrated HGF on intestinal epithelial cell mass and function. Twenty young adult male Sprague-Dawley rats underwent placement of jugular venous catheters connected to subcutaneously placed osmotic minipumps. The rats were divided into four groups based on the contents in the osmotic pump: Group 1 (control, n = 5), normal saline; Group 2 (n = 5), HGF 75 μg/kg/d; Group 3 (n = 5), HGF 150 μg/kg/d; and Group 4 (n = 5), HGF 300 μg/kg/d. After a 14 day infusion, [C14] galactose and [C14] glycine absorption were measured in a 10-cm segment of mid small intestine using an in vivo closed-recirculation technique. Mucosal DNA content and protein content of the same small bowel segment were determined for each group. With all three doses, HGF significantly increased DNA content (P
AB - Hepatocyte growth factor (HGF), originally known to stimulate hepatocyte DNA synthesis, recently has been shown to enhance growth of intestinal epithelial cells in vitro. However, there have been no studies on the effect of HGF on the function of intestinal epithelial cells in vivo. This study was designed to examine the effect of systemically administrated HGF on intestinal epithelial cell mass and function. Twenty young adult male Sprague-Dawley rats underwent placement of jugular venous catheters connected to subcutaneously placed osmotic minipumps. The rats were divided into four groups based on the contents in the osmotic pump: Group 1 (control, n = 5), normal saline; Group 2 (n = 5), HGF 75 μg/kg/d; Group 3 (n = 5), HGF 150 μg/kg/d; and Group 4 (n = 5), HGF 300 μg/kg/d. After a 14 day infusion, [C14] galactose and [C14] glycine absorption were measured in a 10-cm segment of mid small intestine using an in vivo closed-recirculation technique. Mucosal DNA content and protein content of the same small bowel segment were determined for each group. With all three doses, HGF significantly increased DNA content (P
KW - Growth factor
KW - Hepatocyte growth factor
KW - Intestinal function
KW - Intestinal proliferation
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U2 - 10.1016/S0022-3468(97)90384-5
DO - 10.1016/S0022-3468(97)90384-5
M3 - Article
C2 - 9247219
AN - SCOPUS:0030836626
SN - 0022-3468
VL - 32
SP - 991
EP - 994
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 7
ER -