Abstract
OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64% of the HCC+ group and 74% of the HCC- group, 36% of the HCC+ group and 16% of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41% of the HCC+ group and in 24% of the HCC- group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1619-1626 |
| Number of pages | 8 |
| Journal | American Journal of Gastroenterology |
| Volume | 94 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Gastroenterology
Cite this
Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States. / Reid, Andrea E.; Koziel, Margaret James; Aiza, Ignasio; Jeffers, Lennox; Reddy, Rajender; Schiff, Eugene; Lau, Johnson Y N; Dienstag, Jules L.; Liang, T. Jake.
In: American Journal of Gastroenterology, Vol. 94, No. 6, 06.1999, p. 1619-1626.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States
AU - Reid, Andrea E.
AU - Koziel, Margaret James
AU - Aiza, Ignasio
AU - Jeffers, Lennox
AU - Reddy, Rajender
AU - Schiff, Eugene
AU - Lau, Johnson Y N
AU - Dienstag, Jules L.
AU - Liang, T. Jake
PY - 1999/6
Y1 - 1999/6
N2 - OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64% of the HCC+ group and 74% of the HCC- group, 36% of the HCC+ group and 16% of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41% of the HCC+ group and in 24% of the HCC- group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.
AB - OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64% of the HCC+ group and 74% of the HCC- group, 36% of the HCC+ group and 16% of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41% of the HCC+ group and in 24% of the HCC- group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.
UR - http://www.scopus.com/inward/record.url?scp=0033009703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033009703&partnerID=8YFLogxK
U2 - 10.1111/j.1572-0241.1999.01153.x
DO - 10.1111/j.1572-0241.1999.01153.x
M3 - Article
C2 - 10364034
AN - SCOPUS:0033009703
VL - 94
SP - 1619
EP - 1626
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
SN - 0002-9270
IS - 6
ER -