Abstract
Hepatic transplantation into immorally presensitized patients has occasionally been performed without reported accelerated rejection. To study survival of orthotopic hepatic transplants in sensitized recipients a series of studies in rats were performed. Lewis rats sensitized by three successive skin grafts from fully allogeneic ACI strain donors then underwent orthotopic hepatic transplantation from ACI donors. Nine of ten recipients died within 4 hr with bleeding from the liver surface. By comparison, nine unsensitized recipients survived a mean of 10.7±0.5 days before succumbing with cellular rejection. Death of the sensitized recipients was not due to coagulopathy or technical failure. Histological studies of hyperacuteiy rejected livers demonstrated marked hemorrhage, edema, congestion, and necrosis within the hepatic parenchyma. There was a relative lack of cellular infiltrate compared with livers rejected by unsensitized recipients. Immunofluorescent staining showed IgG bound to perivascular tissues and sinusoids, and complement bound to perivascular tissue. Serum from presensitized, but not control, recipients showed a high titer of donor-specific, complement-dependent cytotoxic activity. It is concluded that hyperacute rejection of hepatic transplants can occur in sensitized rats and is mediated by a humoral mechanism. The immunohistopathology of this process is described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8-12 |
| Number of pages | 5 |
| Journal | Transplantation |
| Volume | 43 |
| Issue number | 1 |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Transplantation
- Immunology
Cite this
Hepatic transplantation into sensitized recipients : Demonstration of hyperacute rejection. / Knechtle, Stuart J.; Kolbeck, Peter C.; Tsuchimoto, Shinya; Coundouriotis, Andrew; Sanfilippo, Fred; Bollinger, R. Randal.
In: Transplantation, Vol. 43, No. 1, 1987, p. 8-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatic transplantation into sensitized recipients
T2 - Demonstration of hyperacute rejection
AU - Knechtle, Stuart J.
AU - Kolbeck, Peter C.
AU - Tsuchimoto, Shinya
AU - Coundouriotis, Andrew
AU - Sanfilippo, Fred
AU - Bollinger, R. Randal
PY - 1987
Y1 - 1987
N2 - Hepatic transplantation into immorally presensitized patients has occasionally been performed without reported accelerated rejection. To study survival of orthotopic hepatic transplants in sensitized recipients a series of studies in rats were performed. Lewis rats sensitized by three successive skin grafts from fully allogeneic ACI strain donors then underwent orthotopic hepatic transplantation from ACI donors. Nine of ten recipients died within 4 hr with bleeding from the liver surface. By comparison, nine unsensitized recipients survived a mean of 10.7±0.5 days before succumbing with cellular rejection. Death of the sensitized recipients was not due to coagulopathy or technical failure. Histological studies of hyperacuteiy rejected livers demonstrated marked hemorrhage, edema, congestion, and necrosis within the hepatic parenchyma. There was a relative lack of cellular infiltrate compared with livers rejected by unsensitized recipients. Immunofluorescent staining showed IgG bound to perivascular tissues and sinusoids, and complement bound to perivascular tissue. Serum from presensitized, but not control, recipients showed a high titer of donor-specific, complement-dependent cytotoxic activity. It is concluded that hyperacute rejection of hepatic transplants can occur in sensitized rats and is mediated by a humoral mechanism. The immunohistopathology of this process is described.
AB - Hepatic transplantation into immorally presensitized patients has occasionally been performed without reported accelerated rejection. To study survival of orthotopic hepatic transplants in sensitized recipients a series of studies in rats were performed. Lewis rats sensitized by three successive skin grafts from fully allogeneic ACI strain donors then underwent orthotopic hepatic transplantation from ACI donors. Nine of ten recipients died within 4 hr with bleeding from the liver surface. By comparison, nine unsensitized recipients survived a mean of 10.7±0.5 days before succumbing with cellular rejection. Death of the sensitized recipients was not due to coagulopathy or technical failure. Histological studies of hyperacuteiy rejected livers demonstrated marked hemorrhage, edema, congestion, and necrosis within the hepatic parenchyma. There was a relative lack of cellular infiltrate compared with livers rejected by unsensitized recipients. Immunofluorescent staining showed IgG bound to perivascular tissues and sinusoids, and complement bound to perivascular tissue. Serum from presensitized, but not control, recipients showed a high titer of donor-specific, complement-dependent cytotoxic activity. It is concluded that hyperacute rejection of hepatic transplants can occur in sensitized rats and is mediated by a humoral mechanism. The immunohistopathology of this process is described.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023116650&partnerID=8YFLogxK
M3 - Article
C2 - 3541329
AN - SCOPUS:0023116650
VL - 43
SP - 8
EP - 12
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 1
ER -