Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen

Arnaud Carpentier, Ila Nimgaonkar, Virginia Chu, Yuchen Xia, Zongyi Hu, T. Jake Liang

Research output: Contribution to journalArticle

Abstract

The establishment of protocols to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced pluripotent (iPSC) stem cells into functional hepatocyte-like cells (HLCs) creates new opportunities to study liver metabolism, genetic diseases and infection of hepatotropic viruses (hepatitis B and C viruses) in the context of specific genetic background. While supporting efficient differentiation to HLCs, the published protocols are limited in terms of differentiation into fully mature hepatocytes and in a smaller-well format. This limitation handicaps the application of these cells to high-throughput assays. Here we describe a protocol allowing efficient and consistent hepatic differentiation of hPSCs in 384-well plates into functional hepatocyte-like cells, which remain differentiated for more than 3 weeks. This protocol affords the unique opportunity to miniaturize the hPSC-based differentiation technology and facilitates screening for molecules in modulating liver differentiation, metabolism, genetic network, and response to infection or other external stimuli.

Original languageEnglish (US)
Pages (from-to)640-650
Number of pages11
JournalStem Cell Research
Volume16
Issue number3
DOIs
Publication statusPublished - May 1 2016
Externally publishedYes

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Keywords

  • 384-Well plates
  • Hepatic differentiation
  • High-throughput assay
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

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