TY - JOUR
T1 - Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen
AU - Carpentier, Arnaud
AU - Nimgaonkar, Ila
AU - Chu, Virginia
AU - Xia, Yuchen
AU - Hu, Zongyi
AU - Liang, T. Jake
PY - 2016/5/1
Y1 - 2016/5/1
N2 - The establishment of protocols to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced pluripotent (iPSC) stem cells into functional hepatocyte-like cells (HLCs) creates new opportunities to study liver metabolism, genetic diseases and infection of hepatotropic viruses (hepatitis B and C viruses) in the context of specific genetic background. While supporting efficient differentiation to HLCs, the published protocols are limited in terms of differentiation into fully mature hepatocytes and in a smaller-well format. This limitation handicaps the application of these cells to high-throughput assays. Here we describe a protocol allowing efficient and consistent hepatic differentiation of hPSCs in 384-well plates into functional hepatocyte-like cells, which remain differentiated for more than 3 weeks. This protocol affords the unique opportunity to miniaturize the hPSC-based differentiation technology and facilitates screening for molecules in modulating liver differentiation, metabolism, genetic network, and response to infection or other external stimuli.
AB - The establishment of protocols to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced pluripotent (iPSC) stem cells into functional hepatocyte-like cells (HLCs) creates new opportunities to study liver metabolism, genetic diseases and infection of hepatotropic viruses (hepatitis B and C viruses) in the context of specific genetic background. While supporting efficient differentiation to HLCs, the published protocols are limited in terms of differentiation into fully mature hepatocytes and in a smaller-well format. This limitation handicaps the application of these cells to high-throughput assays. Here we describe a protocol allowing efficient and consistent hepatic differentiation of hPSCs in 384-well plates into functional hepatocyte-like cells, which remain differentiated for more than 3 weeks. This protocol affords the unique opportunity to miniaturize the hPSC-based differentiation technology and facilitates screening for molecules in modulating liver differentiation, metabolism, genetic network, and response to infection or other external stimuli.
KW - 384-Well plates
KW - Hepatic differentiation
KW - High-throughput assay
KW - Pluripotent stem cells
UR - http://www.scopus.com/inward/record.url?scp=84962467029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84962467029&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2016.03.009
DO - 10.1016/j.scr.2016.03.009
M3 - Article
C2 - 27062358
AN - SCOPUS:84962467029
SN - 1873-5061
VL - 16
SP - 640
EP - 650
JO - Stem Cell Research
JF - Stem Cell Research
IS - 3
ER -