Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria

Ekaterina Y. Lukianova-Hleb; Kelly M. Campbell; Pamela E. Constantinou; Janet Braam; John S. Olson; Russell E. Ware; David J. Sullivan; Dmitri O. Lapotko

doi:10.1073/pnas.1316253111

Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria

Ekaterina Y. Lukianova-Hleb, Kelly M. Campbell, Pamela E. Constantinou, Janet Braam, John S. Olson, Russell E. Ware, David J. Sullivan, Dmitri O. Lapotko

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Successful diagnosis, screening, and elimination of malaria critically depend on rapid and sensitive detection of this dangerous infection, preferably transdermally and without sophisticated reagents or blood drawing. Such diagnostic methods are not currently available. Here we show that the high optical absorbance and nanosize of endogenous heme nanoparticles called "hemozoin," a unique component of all blood-stage malaria parasites, generates a transient vapor nanobubble around hemozoin in response to a short and safe near-infrared picosecond laser pulse. The acoustic signals of these malaria-specific nanobubbles provided transdermal noninvasive and rapid detection of a malaria infection as low as 0.00034% in animals without using any reagents or drawing blood. These on-demand transient events have no analogs among current malaria markers and probes, can detect and screen malaria in seconds, and can be realized as a compact, easy-to-use, inexpensive, and safe field technology.

Original language	English (US)
Pages (from-to)	900-905
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	111
Issue number	3
DOIs	https://doi.org/10.1073/pnas.1316253111
State	Published - Jan 21 2014

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Lukianova-Hleb, E. Y., Campbell, K. M., Constantinou, P. E., Braam, J., Olson, J. S., Ware, R. E., Sullivan, D. J., & Lapotko, D. O. (2014). Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria. Proceedings of the National Academy of Sciences of the United States of America, 111(3), 900-905. https://doi.org/10.1073/pnas.1316253111

Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria. / Lukianova-Hleb, Ekaterina Y.; Campbell, Kelly M.; Constantinou, Pamela E.; Braam, Janet; Olson, John S.; Ware, Russell E.; Sullivan, David J.; Lapotko, Dmitri O.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 3, 21.01.2014, p. 900-905.

Research output: Contribution to journal › Article › peer-review

Lukianova-Hleb, Ekaterina Y. ; Campbell, Kelly M. ; Constantinou, Pamela E. ; Braam, Janet ; Olson, John S. ; Ware, Russell E. ; Sullivan, David J. ; Lapotko, Dmitri O. / Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 3. pp. 900-905.

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abstract = "Successful diagnosis, screening, and elimination of malaria critically depend on rapid and sensitive detection of this dangerous infection, preferably transdermally and without sophisticated reagents or blood drawing. Such diagnostic methods are not currently available. Here we show that the high optical absorbance and nanosize of endogenous heme nanoparticles called {"}hemozoin,{"} a unique component of all blood-stage malaria parasites, generates a transient vapor nanobubble around hemozoin in response to a short and safe near-infrared picosecond laser pulse. The acoustic signals of these malaria-specific nanobubbles provided transdermal noninvasive and rapid detection of a malaria infection as low as 0.00034% in animals without using any reagents or drawing blood. These on-demand transient events have no analogs among current malaria markers and probes, can detect and screen malaria in seconds, and can be realized as a compact, easy-to-use, inexpensive, and safe field technology.",

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