Hemophiliacs With HIV Antibody Are Actively Infected

J. Brooks Jackson, Kim J. Sannerud, Jocelyn S. Hopsicker, Shirley Y. Kwok, J. Roger Edson, Henry H. Balfour

Research output: Contribution to journalArticle

Abstract

Cultures of peripheral blood mononuclear cells for human immunodeficiency virus type 1 (HIV-1) and assays for the p24 antigen were performed for a group of 75 unselected hemophiliacs to determine whether patients positive for HIV-1 antibody are actively infected rather than immunized by viral proteins in non— heat-treated factor VIII or IX concentrates. Fifty-six (75%) of the 75 hemophiliacs were antibody positive and 55 (98%) of the 56 with antibodies also had positive cultures. The one culture-negative individual had detectable HIV-1 proviral DNA sequences in three separate samples of peripheral blood mononuclear cell DNA, as detected by a polymerase chain reaction assay. Detection of serum p24 antigen and the time to development of a positive culture were significantly more frequent and shorter, respectively, in symptomatic vs asymptomatic patients. None of the 19 hemophiliacs negative for HIV-1 antibody had positive cultures, detectable p24 serum antigen, or symptoms of HIV-1 infection. Moreover, latent HIV-1 infection was not detected in 16 female sexual partners of hemophiliacs positive for HIV-1 antibody using Western blot testing, assays for p24 antigen, HIV-1 cultures, and polymerase chain reaction assays, despite repeated unprotected sexual exposure. We conclude that antibody-positive hemophiliacs have been actively infected by HIV-1 and that a long period of latent HIV-1 infection prior to overt seroconversion is unlikely.

Original languageEnglish (US)
Pages (from-to)2236-2239
Number of pages4
JournalJournal of the American Medical Association
Volume260
Issue number15
DOIs
StatePublished - Oct 21 1988
Externally publishedYes

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HIV Antibodies
HIV-1
Antibodies
Virus Diseases
Antigens
Blood Cells
Polymerase Chain Reaction
Factor IX
Sexual Partners
Factor VIII
Viral Proteins
Serum
Hot Temperature
Western Blotting

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Jackson, J. B., Sannerud, K. J., Hopsicker, J. S., Kwok, S. Y., Edson, J. R., & Balfour, H. H. (1988). Hemophiliacs With HIV Antibody Are Actively Infected. Journal of the American Medical Association, 260(15), 2236-2239. https://doi.org/10.1001/jama.1988.03410150084035

Hemophiliacs With HIV Antibody Are Actively Infected. / Jackson, J. Brooks; Sannerud, Kim J.; Hopsicker, Jocelyn S.; Kwok, Shirley Y.; Edson, J. Roger; Balfour, Henry H.

In: Journal of the American Medical Association, Vol. 260, No. 15, 21.10.1988, p. 2236-2239.

Research output: Contribution to journalArticle

Jackson, JB, Sannerud, KJ, Hopsicker, JS, Kwok, SY, Edson, JR & Balfour, HH 1988, 'Hemophiliacs With HIV Antibody Are Actively Infected', Journal of the American Medical Association, vol. 260, no. 15, pp. 2236-2239. https://doi.org/10.1001/jama.1988.03410150084035
Jackson JB, Sannerud KJ, Hopsicker JS, Kwok SY, Edson JR, Balfour HH. Hemophiliacs With HIV Antibody Are Actively Infected. Journal of the American Medical Association. 1988 Oct 21;260(15):2236-2239. https://doi.org/10.1001/jama.1988.03410150084035
Jackson, J. Brooks ; Sannerud, Kim J. ; Hopsicker, Jocelyn S. ; Kwok, Shirley Y. ; Edson, J. Roger ; Balfour, Henry H. / Hemophiliacs With HIV Antibody Are Actively Infected. In: Journal of the American Medical Association. 1988 ; Vol. 260, No. 15. pp. 2236-2239.
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