Hemolysis of 40% hematocrit, Albunexρ-supplemented human erythrocytes by pulsed ultrasound: Frequency, acoustic pressure and pulse length dependence

Andrew A. Brayman, Peggy L. Strickler, Huong Luan, Susan L. Barned, Carol H. Raeman, Christopher Cox, Morton W. Miller

Research output: Contribution to journalArticle


The dependence of hemolysis produced by pulsed ultrasound on ultrasound frequency, acoustic pressure and pulse length was explored. Human erythrocytes (40% hematocrit; in Albunex ®-supplemented autologous plasma) were exposed (60 s) to 20 or 200 μs pulses of ultrasound at frequencies of 1.02, 2.24 or 3.46 MHz and at peak negative pressures [P-] ranging from 0.0 to ~3.0 MPa in 0.5 MPa increments. The duty factor was 0.01. At each frequency, hemolysis increased with increasing acoustic pressure and depended weakly on pulse duration. At relatively high acoustic pressures, hemolysis depended strongly on ultrasound frequency; at lower pressures, the frequency dependence was weaker. The potential clinical significance of ultrasonic hemolysis is discussed.

Original languageEnglish (US)
Pages (from-to)1237-1250
Number of pages14
JournalUltrasound in Medicine and Biology
Issue number8
Publication statusPublished - 1997
Externally publishedYes



  • Acoustic cavitation
  • Albunexρ
  • Hemolysis
  • Pulsed ultrasound
  • Ultrasound bioeffects

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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