Hemolysis in vivo from exposure to pulsed ultrasound

Diane Dalecki, C. H. Raeman, S. Z. Child, C. Cox, C. W. Francis, R. S. Meltzer, E. L. Carstensen

Research output: Contribution to journalArticlepeer-review

Abstract

Ultrasonically induced hemolysis in vivo was studied when a commercial ultrasound contrast agent, Albunex®, was present in the blood. Murine hearts were exposed for 5 min at either 1.15 or 2.35 MHz with a pulse length of 10 μs and pulse repetition frequency of 100 Hz. During the exposure period, four boluses of Albunex® were injected into a tail vein for a total of ~ 0.1 mL of Albunex®. Following exposure, blood was collected by heart puncture and centrifuged, and the plasma was analyzed for hemoglo bin concentration. With Albunex® present in the blood, the threshold for hemolysis at 1.15 MHz was 3.0 ± 0.8 MPa (mean ± SD) peak positive pressure (~ 1.9 MPa negative pressure, ~ 180 W cm-2 pulse average intensity). For the highest exposure levels (10 MPa peak positive pressure at the surface of the animal), the mean value for hemolysis was ~ 4% at 1.15 MHz and 0.46% at 2.35 MHz, i.e., the threshold at 2.35 MHz is > 10 MPa peak positive pressure. In contrast, hemolysis in control mice receiving saline injections at 10 MPa or sham-exposed (0 MPa) mice receiving Albunex® was ~ 0.4%.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalUltrasound in Medicine and Biology
Volume23
Issue number2
DOIs
StatePublished - Apr 21 1997
Externally publishedYes

Keywords

  • Bioeffect
  • Contrast agent
  • Hemolysis
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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