Hemolysin from Shiga toxin-negative Escherichia coli O26 strains injures microvascular endothelium

Thomas Aldick, Martina Bielaszewska, Wenlan Zhang, Jens Brockmeyer, Herbert Schmidt, Alexander W. Friedrich, Kwang S. Kim, M. Alexander Schmidt, Helge Karch

Research output: Contribution to journalArticle

Abstract

We identified Shiga toxin gene (stx)-negative Escherichia coli O26:H11 and O26:NM (nonmotile) strains as the only pathogens in the stools of five patients with hemolytic-uremic syndrome (HUS). Because the absence of stx in E. coli associated with HUS is unusual, we examined the strains for potential virulence factors and interactions with microvascular endothelial cells which are the major targets affected during HUS. All five isolates possessed the enterohemorrhagic E. coli (EHEC)-hlyA gene encoding EHEC hemolysin (EHEC-Hly), expressed the enterohemolytic phenotype, and were cytotoxic, in dose- and time-dependent manners, to human brain microvascular endothelial cells (HBMECs). Significantly reduced cytotoxicity in an EHEC-Hly-negative spontaneous derivative of one of these strains, and a dose- and time-dependent cytotoxicity of recombinant E. coli O26 EHEC-Hly to HBMECs, suggest that the endothelial cytotoxicity of these strains was mediated by EHEC-Hly. The toxicity of EHEC-Hly to microvascular endothelial cells plausibly contributes to the virulence of the stx-negative E. coli O26 strains and to the pathogenesis of HUS.

Original languageEnglish (US)
Pages (from-to)282-290
Number of pages9
JournalMicrobes and Infection
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • Adherence
  • Cytotoxicity
  • Enterohemorrhagic E. coli hemolysin
  • Escherichia coli O26
  • Hemolytic-Uremic syndrome
  • Microvascular endothelial cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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  • Cite this

    Aldick, T., Bielaszewska, M., Zhang, W., Brockmeyer, J., Schmidt, H., Friedrich, A. W., Kim, K. S., Schmidt, M. A., & Karch, H. (2007). Hemolysin from Shiga toxin-negative Escherichia coli O26 strains injures microvascular endothelium. Microbes and Infection, 9(3), 282-290. https://doi.org/10.1016/j.micinf.2006.12.001