Hemoglobin tetramers stabilized with polyaspirins

Enrico Buccl, Clara Fronticelli, Anna Razynska, Valeria Militello, Raymond C Koehler, Barbara Urbaltls

Research output: Contribution to journalArticle

Abstract

Organic acids activated by esterification with 3,5-dibromosalicylate react preferentially either with the 682 lysines or the a99 lysines of hemoglobin. The versatility and site specificity of these polysapirins and the usage of both human and bovine hemoglobins allowed the construction of a family of oxygen carriers with various P5O ranging from 10 to 50 mmHg. These derivatives are obtained in pure homogeneous form by column chromatography. They are stabilized tetramers where the dissociation into dimers is inhibited. The latest addition is Tri-(3,5,dibromosallcyl)-benzenetricarboxylate, which crosslinks both human and bovine hemoglobin across the 0 subunits, decreasing the oxygen affinity of both proteins. The crosslinked hemoglobins have a normal Bohr effect, more expanded in the alkaline region, and are sensitive to chlorides but not to polyphosphates. Solutions of stabilized tetramers, infused into rats or cats up to 25-50X blood replacement, do not produce altered renal and cardiac function. In the cat isovolemic hemodilution increases cerebral flow in controls treated with albumin solutions, when an oxygen carier is used the cerbral flow remains normal.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalBiomaterials, Artificial Cells, and Immobilization Biotechnology
Volume20
Issue number4 --Feb
DOIs
StatePublished - 1992
Externally publishedYes

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Hemoglobin
Hemoglobins
Oxygen
Lysine
Cats
Polyphosphates
Column chromatography
Hemodilution
Esterification
Organic acids
Dimers
Chromatography
Rats
Chlorides
Albumins
Blood
Derivatives
Proteins
Kidney
Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hemoglobin tetramers stabilized with polyaspirins. / Buccl, Enrico; Fronticelli, Clara; Razynska, Anna; Militello, Valeria; Koehler, Raymond C; Urbaltls, Barbara.

In: Biomaterials, Artificial Cells, and Immobilization Biotechnology, Vol. 20, No. 4 --Feb, 1992, p. 243-252.

Research output: Contribution to journalArticle

Buccl, Enrico ; Fronticelli, Clara ; Razynska, Anna ; Militello, Valeria ; Koehler, Raymond C ; Urbaltls, Barbara. / Hemoglobin tetramers stabilized with polyaspirins. In: Biomaterials, Artificial Cells, and Immobilization Biotechnology. 1992 ; Vol. 20, No. 4 --Feb. pp. 243-252.
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