Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft

Kazuo Shirakawa, Hisataka Kobayashi, Yuji Heike, Satomi Kawamoto, Martin W. Brechbiel, Satomi Kawamoto, Toshihiko Iwanaga, Fumio Konishi, Masaaki Terada, Hiro Wakasugi

Research output: Contribution to journalArticle

Abstract

In the present study, we examined hemodynamics in vasculogenic mimicry (VM) and angiogenesis of inflammatory breast cancer (IBC) xenografts (WIBC-9), having previously reported on the unique histological features and molecular basis of these processes (K. Shirakawa et al., Cancer Res., 61: 445-451, 2001). Histologically, the WIBC-9 xenografts exhibited invasive ductal carcinoma with a hypervascular structure (angiogenesis) in the tumor margin and VM without endothelial cells, central necrosis, or fibrosis in the tumor center. Results of molecular analysis indicated that WIBC-9 had a vasculogenic phenotype, including expression of Flt-1 and Tie-2. Comparison of WIBC-9 with an established non-IBC xenograft (MC-5), using time-coursed dynamic micromagnetic resonance angiography analysis (with our newly developed intravascular macromolecular magnetic resonance imaging contrast agent), electromicroscopy, and immunohistochemistry, demonstrated blood flow and a VM-angiogenesis junction in the central area of the WIBC-9 tumor. It has previously been considered impossible to prove a connection between VM and angiogenesis using angiography, because there are no intravascular macromolecular magnetic resonance imaging contrast agents that do not exhibit significant leakage through the vascular wall. In the present study, laser-captured microdissection was performed in regions of WIBC-9 tumors that exhibited VM without endothelial cells, central necrosis, or fibrosis, revealing expression of human-Flt-1 and human-Tie2 and the absence of human-CD31, human-endothelin B receptor, and human-thrombin receptor. These facts led us to hypothesize that the VM of WIBC-9 involves hemodynamics that serve to feed WIBC-9 cells, and this in turn suggests a connection between VM and angiogenesis.

Original languageEnglish (US)
Pages (from-to)560-566
Number of pages7
JournalCancer Research
Volume62
Issue number2
StatePublished - Jan 15 2002
Externally publishedYes

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Inflammatory Breast Neoplasms
Heterografts
Hemodynamics
Neoplasms
Contrast Media
Angiography
Fibrosis
Necrosis
Endothelial Cells
Magnetic Resonance Imaging
Endothelin B Receptors
Thrombin Receptors
Microdissection
Ductal Carcinoma
Blood Vessels
Lasers
Immunohistochemistry
Breast Neoplasms
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Shirakawa, K., Kobayashi, H., Heike, Y., Kawamoto, S., Brechbiel, M. W., Kawamoto, S., ... Wakasugi, H. (2002). Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft. Cancer Research, 62(2), 560-566.

Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft. / Shirakawa, Kazuo; Kobayashi, Hisataka; Heike, Yuji; Kawamoto, Satomi; Brechbiel, Martin W.; Kawamoto, Satomi; Iwanaga, Toshihiko; Konishi, Fumio; Terada, Masaaki; Wakasugi, Hiro.

In: Cancer Research, Vol. 62, No. 2, 15.01.2002, p. 560-566.

Research output: Contribution to journalArticle

Shirakawa, K, Kobayashi, H, Heike, Y, Kawamoto, S, Brechbiel, MW, Kawamoto, S, Iwanaga, T, Konishi, F, Terada, M & Wakasugi, H 2002, 'Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft', Cancer Research, vol. 62, no. 2, pp. 560-566.
Shirakawa K, Kobayashi H, Heike Y, Kawamoto S, Brechbiel MW, Kawamoto S et al. Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft. Cancer Research. 2002 Jan 15;62(2):560-566.
Shirakawa, Kazuo ; Kobayashi, Hisataka ; Heike, Yuji ; Kawamoto, Satomi ; Brechbiel, Martin W. ; Kawamoto, Satomi ; Iwanaga, Toshihiko ; Konishi, Fumio ; Terada, Masaaki ; Wakasugi, Hiro. / Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft. In: Cancer Research. 2002 ; Vol. 62, No. 2. pp. 560-566.
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