TY - JOUR
T1 - Heme signaling impacts global gene expression, immunity and dengue virus infectivity in Aedes aegypti
AU - Bottino-Rojas, Vanessa
AU - Talyuli, Octávio A.C.
AU - Jupatanakul, Natapong
AU - Sim, Shuzhen
AU - Dimopoulos, George
AU - Venancio, Thiago M.
AU - Bahia, Ana C.
AU - Sorgine, Marcos H.
AU - Oliveira, Pedro L.
AU - Paiva-Silva, Gabriela O.
N1 - Publisher Copyright:
Copyright: © 2015 Bottino-Rojas et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2015/8/14
Y1 - 2015/8/14
N2 - Blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. Heme is a pro-oxidant that influences a variety of cellular processes. We performed a global analysis of heme-regulated Aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. We observed an iron- and reactive oxygen species (ROS)-independent signaling induced by heme that comprised genes related to redox metabolism. By modulating the abundance of these transcripts, heme possibly acts as a danger signaling molecule. Furthermore, heme triggered critical changes in the expression of energy metabolism and immune response genes, altering the susceptibility towards bacteria and dengue virus. These findings seem to have implications on the adaptation of mosquitoes to hematophagy and consequently on their ability to transmit diseases. Altogether, these results may also contribute to the understanding of heme cell biology in eukaryotic cells.
AB - Blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. Heme is a pro-oxidant that influences a variety of cellular processes. We performed a global analysis of heme-regulated Aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. We observed an iron- and reactive oxygen species (ROS)-independent signaling induced by heme that comprised genes related to redox metabolism. By modulating the abundance of these transcripts, heme possibly acts as a danger signaling molecule. Furthermore, heme triggered critical changes in the expression of energy metabolism and immune response genes, altering the susceptibility towards bacteria and dengue virus. These findings seem to have implications on the adaptation of mosquitoes to hematophagy and consequently on their ability to transmit diseases. Altogether, these results may also contribute to the understanding of heme cell biology in eukaryotic cells.
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U2 - 10.1371/journal.pone.0135985
DO - 10.1371/journal.pone.0135985
M3 - Article
C2 - 26275150
AN - SCOPUS:84942905523
SN - 1932-6203
VL - 10
JO - PloS one
JF - PloS one
IS - 8
M1 - e0135985
ER -