Heme oxygenase-1 protects Corexit 9500ainduced respiratory epithelial injury across species

Fu Jun Li, Ryan N. Duggal, Octavio M. Oliva, Suman Karki, Ranu Surolia, Zheng Wang, R. Douglas Watson, Victor J. Thannickal, Mickie Powell, Stephen Watts, Tejaswini Kulkarni, Hitesh Batra, Subhashini Bolisetty, Anupam Agarwal, Veena B. Antony

Research output: Contribution to journalArticle

Abstract

The effects of Corexit 9500A (CE) on respiratory epithelial surfaces of terrestrial mammals and marine animals are largely unknown. This study investigated the role of CE-induced heme oxygenase-1 (HO-1), a cytoprotective enzyme with anti-apoptotic and antioxidant activity, in human bronchial airway epithelium and the gills of exposed aquatic animals.We evaluated CE-mediated alterations in human airway epithelial cells, mice lungs and gills from zebrafish and blue crabs. Our results demonstrated that CE induced an increase in gill epithelial edema and human epithelialmonolayer permeability, suggesting an acute injury caused by CE exposure. CE induced the expression of HO-1 as well as C-reactive protein (CRP) and NADPH oxidase 4(NOX4), which are associated with ROS production. Importantly, CE induced caspase-3 activation and subsequent apoptosis of epithelial cells. The expression of the intercellular junctional proteins, such as tight junction proteins occludin, zonula occludens (ZO-1), ZO-2 and adherens junctional proteins E-cadherin and Focal Adhesion Kinase (FAK), were remarkably inhibited by CE, suggesting that these proteins are involved in CE-induced increased permeability and subsequent apoptosis. The cytoskeletal protein F-actin was also disrupted by CE. Treatment with carbon monoxide releasing molecule-2 (CORM-2) significantly inhibited CE-induced ROS production, while the addition of HO-1 inhibitor, significantly increased CE-induced ROS production and apoptosis, suggesting a protective role of HO-1 or its reaction product, CO, in CE-induced apoptosis. Using HO-1 knockoutmice, we further demonstrated that HO-1 protected against CE-induced inflammation and cellular apoptosis and corrected CE-mediated inhibition of E-cadherin and FAK. These observations suggest that CE activates CRP and NOX4-mediated ROS production, alters permeability by inhibition of junctional proteins, and leads to caspase-3 dependent apoptosis of epithelial cells, while HO-1 and its reaction products protect against oxidative stress and apoptosis.

Original languageEnglish (US)
Article numbere0122275
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 2 2015
Externally publishedYes

Fingerprint

heme oxygenase (biliverdin-producing)
Heme Oxygenase-1
apoptosis
Apoptosis
Wounds and Injuries
non-specific protein-tyrosine kinase
Permeability
Focal Adhesion Protein-Tyrosine Kinases
gills
permeability
epithelial cells
tight junctions
cadherins
NADPH Oxidase
Epithelial Cells
C-reactive protein
caspase-3
Cadherins
Carbon Monoxide
Reaction products

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Li, F. J., Duggal, R. N., Oliva, O. M., Karki, S., Surolia, R., Wang, Z., ... Antony, V. B. (2015). Heme oxygenase-1 protects Corexit 9500ainduced respiratory epithelial injury across species. PLoS One, 10(4), [e0122275]. https://doi.org/10.1371/journal.pone.0122275

Heme oxygenase-1 protects Corexit 9500ainduced respiratory epithelial injury across species. / Li, Fu Jun; Duggal, Ryan N.; Oliva, Octavio M.; Karki, Suman; Surolia, Ranu; Wang, Zheng; Watson, R. Douglas; Thannickal, Victor J.; Powell, Mickie; Watts, Stephen; Kulkarni, Tejaswini; Batra, Hitesh; Bolisetty, Subhashini; Agarwal, Anupam; Antony, Veena B.

In: PLoS One, Vol. 10, No. 4, e0122275, 02.04.2015.

Research output: Contribution to journalArticle

Li, FJ, Duggal, RN, Oliva, OM, Karki, S, Surolia, R, Wang, Z, Watson, RD, Thannickal, VJ, Powell, M, Watts, S, Kulkarni, T, Batra, H, Bolisetty, S, Agarwal, A & Antony, VB 2015, 'Heme oxygenase-1 protects Corexit 9500ainduced respiratory epithelial injury across species', PLoS One, vol. 10, no. 4, e0122275. https://doi.org/10.1371/journal.pone.0122275
Li, Fu Jun ; Duggal, Ryan N. ; Oliva, Octavio M. ; Karki, Suman ; Surolia, Ranu ; Wang, Zheng ; Watson, R. Douglas ; Thannickal, Victor J. ; Powell, Mickie ; Watts, Stephen ; Kulkarni, Tejaswini ; Batra, Hitesh ; Bolisetty, Subhashini ; Agarwal, Anupam ; Antony, Veena B. / Heme oxygenase-1 protects Corexit 9500ainduced respiratory epithelial injury across species. In: PLoS One. 2015 ; Vol. 10, No. 4.
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