Hemagglutinin protein is a primary target of the measles virus-specific HLA-A2-restricted CD8+ T cell response during measles and after vaccination

Martin O. Ota, Zaza Ndhlovu, Sang Kon Oh, Sucheep Piyasirisilp, Jay A. Berzofsky, William J. Moss, Diane E. Griffin

Research output: Contribution to journalArticlepeer-review

Abstract

To characterize the measles virus (MV)-specific T cell responses important for evaluation of measles vaccines, human leukocyte antigen (HLA)-A2-positive and -negative adults immunized with measles-mumps-rubella vaccine were studied. Both groups developed increases in antibody and in interferon (IFN)-γ-producing cells in response to pooled hemagglutinin (H) and fusion peptides. HLA-A2-binding peptides were predicted for all MV-encoded proteins and confirmed by T2 cell stabilization. Twenty-nine peptides were tested, and 19 (6 from H) stimulated increased IFN-γ secretion in a majority of vaccinees. Peptide-loaded HLA-A2 tetramers or immunoglobulin dimers documented MV-specific CD8+ T cell responses after vaccination and during measles and confirmed new A2 epitopes in H (250-259 and 516-525 aa) and matrix (M; 50-58 aa) protein and previously described epitopes in H (30-38 aa), M (211-219 aa), and nonstructural protein C (84-92 aa). No single peptide dominated the response. We conclude that H is an important stimulus for CD8 + T cell as well as for antibody responses in HLA-A2-positive individuals.

Original languageEnglish (US)
Pages (from-to)1799-1807
Number of pages9
JournalJournal of Infectious Diseases
Volume195
Issue number12
DOIs
StatePublished - Jun 15 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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