Helminth genome analysis: The current status of the filarial and schistosome genome projects

S. A. Williams, D. A. Johnston, Martin Aslett, LouAnn Bierwert, Mark L. Blaxter, Jennifer Daub, Jeremy Foster, Mehul Ganatra, David Guiliano, Susan Haynes, Kunthala Jayaraman, Ibrahim H. Kamal, K. Kannan, Sandra J. Laney, Wen Li, Michelle Lizotte-Waniewski, Wenhong Lu, Nithyakalyani Raghavan, Reda M R Ramzy, R. V. RaoLori Saunders, Alan Leroy Scott, Barton Slatko, Taniawati Supali, Jennifer Ware, Vasco Azevedo, Paul Brindley, Guilherme Correa De Oliviera, Zheng Feng, Gloria R. Franco, Hiro Hirai, Amr Karim, Philip T. LoVerde, Don McManus, Joe Merrick, Ray Pierce, Elida Rabelo, David Rollinson, Mohamed Saber, David L. Williams

Research output: Contribution to journalArticle

Abstract

Genome projects for the parasitic helminths Brugia malayi (a representative filarial nematode) and Schistosoma were initiated in 1995 by the World Health Organization with the ultimate objectives of identifying new vaccine candidates and drug targets and of developing low resolution genome maps. Because no genetic maps are available, and very few genes have been characterized from either parasite group, the first goal of both Initiatives has been to catalogue new genes for future placement on chromosome and physical maps. These genes have been identified by the expressed sequence tag (EST) approach, utilising cDNA libraries constructed from diverse life cycle stages. To date, the Initiatives have deposited over 16000 Brugia ESTs and nearly 8000 Schistosoma ESTs in Genbank's dbEST database, corresponding to 6000 and over 3600 genes respectively (33% of Brugia's estimated gene compliment, 18-24% of that of Schistosoma). Large fragment, genomic libraries have been constructed in BAC and YAC vectors for studies of genomic organization and for physical and chromosome mapping, and public, hypertext genomic databases have been established to facilitate data access. We present a summary of progress within the helminth genome initiatives and give several examples of important gene discoveries and future applications of these data.

Original languageEnglish (US)
JournalParasitology
Volume118
Issue numberSUPPL.
StatePublished - 1999
Externally publishedYes

Fingerprint

Helminth Genome
Schistosoma
helminths
Genome
Expressed Sequence Tags
Brugia
genome
Genes
genes
physical chromosome mapping
Physical Chromosome Mapping
chromosome mapping
Hypermedia
Brugia malayi
Databases
Genomic Library
Helminths
Nucleic Acid Databases
Genetic Association Studies
Life Cycle Stages

Keywords

  • Brugia
  • EST
  • Expressed sequence tag
  • Filarial nematode
  • Gene discovery
  • Genome analysis
  • Genome project
  • Informatics
  • Physical mapping
  • Schistosoma

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Williams, S. A., Johnston, D. A., Aslett, M., Bierwert, L., Blaxter, M. L., Daub, J., ... Williams, D. L. (1999). Helminth genome analysis: The current status of the filarial and schistosome genome projects. Parasitology, 118(SUPPL.).

Helminth genome analysis : The current status of the filarial and schistosome genome projects. / Williams, S. A.; Johnston, D. A.; Aslett, Martin; Bierwert, LouAnn; Blaxter, Mark L.; Daub, Jennifer; Foster, Jeremy; Ganatra, Mehul; Guiliano, David; Haynes, Susan; Jayaraman, Kunthala; Kamal, Ibrahim H.; Kannan, K.; Laney, Sandra J.; Li, Wen; Lizotte-Waniewski, Michelle; Lu, Wenhong; Raghavan, Nithyakalyani; Ramzy, Reda M R; Rao, R. V.; Saunders, Lori; Scott, Alan Leroy; Slatko, Barton; Supali, Taniawati; Ware, Jennifer; Azevedo, Vasco; Brindley, Paul; Correa De Oliviera, Guilherme; Feng, Zheng; Franco, Gloria R.; Hirai, Hiro; Karim, Amr; LoVerde, Philip T.; McManus, Don; Merrick, Joe; Pierce, Ray; Rabelo, Elida; Rollinson, David; Saber, Mohamed; Williams, David L.

In: Parasitology, Vol. 118, No. SUPPL., 1999.

Research output: Contribution to journalArticle

Williams, SA, Johnston, DA, Aslett, M, Bierwert, L, Blaxter, ML, Daub, J, Foster, J, Ganatra, M, Guiliano, D, Haynes, S, Jayaraman, K, Kamal, IH, Kannan, K, Laney, SJ, Li, W, Lizotte-Waniewski, M, Lu, W, Raghavan, N, Ramzy, RMR, Rao, RV, Saunders, L, Scott, AL, Slatko, B, Supali, T, Ware, J, Azevedo, V, Brindley, P, Correa De Oliviera, G, Feng, Z, Franco, GR, Hirai, H, Karim, A, LoVerde, PT, McManus, D, Merrick, J, Pierce, R, Rabelo, E, Rollinson, D, Saber, M & Williams, DL 1999, 'Helminth genome analysis: The current status of the filarial and schistosome genome projects', Parasitology, vol. 118, no. SUPPL..
Williams SA, Johnston DA, Aslett M, Bierwert L, Blaxter ML, Daub J et al. Helminth genome analysis: The current status of the filarial and schistosome genome projects. Parasitology. 1999;118(SUPPL.).
Williams, S. A. ; Johnston, D. A. ; Aslett, Martin ; Bierwert, LouAnn ; Blaxter, Mark L. ; Daub, Jennifer ; Foster, Jeremy ; Ganatra, Mehul ; Guiliano, David ; Haynes, Susan ; Jayaraman, Kunthala ; Kamal, Ibrahim H. ; Kannan, K. ; Laney, Sandra J. ; Li, Wen ; Lizotte-Waniewski, Michelle ; Lu, Wenhong ; Raghavan, Nithyakalyani ; Ramzy, Reda M R ; Rao, R. V. ; Saunders, Lori ; Scott, Alan Leroy ; Slatko, Barton ; Supali, Taniawati ; Ware, Jennifer ; Azevedo, Vasco ; Brindley, Paul ; Correa De Oliviera, Guilherme ; Feng, Zheng ; Franco, Gloria R. ; Hirai, Hiro ; Karim, Amr ; LoVerde, Philip T. ; McManus, Don ; Merrick, Joe ; Pierce, Ray ; Rabelo, Elida ; Rollinson, David ; Saber, Mohamed ; Williams, David L. / Helminth genome analysis : The current status of the filarial and schistosome genome projects. In: Parasitology. 1999 ; Vol. 118, No. SUPPL.
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abstract = "Genome projects for the parasitic helminths Brugia malayi (a representative filarial nematode) and Schistosoma were initiated in 1995 by the World Health Organization with the ultimate objectives of identifying new vaccine candidates and drug targets and of developing low resolution genome maps. Because no genetic maps are available, and very few genes have been characterized from either parasite group, the first goal of both Initiatives has been to catalogue new genes for future placement on chromosome and physical maps. These genes have been identified by the expressed sequence tag (EST) approach, utilising cDNA libraries constructed from diverse life cycle stages. To date, the Initiatives have deposited over 16000 Brugia ESTs and nearly 8000 Schistosoma ESTs in Genbank's dbEST database, corresponding to 6000 and over 3600 genes respectively (33{\%} of Brugia's estimated gene compliment, 18-24{\%} of that of Schistosoma). Large fragment, genomic libraries have been constructed in BAC and YAC vectors for studies of genomic organization and for physical and chromosome mapping, and public, hypertext genomic databases have been established to facilitate data access. We present a summary of progress within the helminth genome initiatives and give several examples of important gene discoveries and future applications of these data.",
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AU - Williams, S. A.

AU - Johnston, D. A.

AU - Aslett, Martin

AU - Bierwert, LouAnn

AU - Blaxter, Mark L.

AU - Daub, Jennifer

AU - Foster, Jeremy

AU - Ganatra, Mehul

AU - Guiliano, David

AU - Haynes, Susan

AU - Jayaraman, Kunthala

AU - Kamal, Ibrahim H.

AU - Kannan, K.

AU - Laney, Sandra J.

AU - Li, Wen

AU - Lizotte-Waniewski, Michelle

AU - Lu, Wenhong

AU - Raghavan, Nithyakalyani

AU - Ramzy, Reda M R

AU - Rao, R. V.

AU - Saunders, Lori

AU - Scott, Alan Leroy

AU - Slatko, Barton

AU - Supali, Taniawati

AU - Ware, Jennifer

AU - Azevedo, Vasco

AU - Brindley, Paul

AU - Correa De Oliviera, Guilherme

AU - Feng, Zheng

AU - Franco, Gloria R.

AU - Hirai, Hiro

AU - Karim, Amr

AU - LoVerde, Philip T.

AU - McManus, Don

AU - Merrick, Joe

AU - Pierce, Ray

AU - Rabelo, Elida

AU - Rollinson, David

AU - Saber, Mohamed

AU - Williams, David L.

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