Helicobacter pylori urease activity is toxic to human gastric epithelial cells

D. T. Smoot, H. L T Mobley, G. R. Chippendale, J. F. Lewison, J. H. Resau

Research output: Contribution to journalArticle

Abstract

A human gastric adenocarcinoma cell line was used to evaluate the contribution of urease from Helicobacter (formerly Campylobacter) pylori to its cytotoxicity. Gastric cells cultured in medium supplemented with 20 mM urea were exposed to 5 x 106 CFU of H. pylori per ml with or without the addition of a urease inhibitor, acetohydroxamic acid. Viabilities of cells exposed to H. pylori for 2, 24, and 48 h, assessed by incorporation of neutral red dye, were 60, 27, and 16%, respectively; however, the viabilities of cells exposed to both H. pylori and acetohydroxamic acid were 92, 46, and 20% after 2, 24, and 48 h, respectively, (P <0.001). Therefore, the urease activity of H. pylori may play an important role in its pathogenicity, and inhibition of this enzyme activity may have therapeutic potential.

Original languageEnglish (US)
Pages (from-to)1992-1994
Number of pages3
JournalInfection and Immunity
Volume58
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

Urease
Poisons
Helicobacter pylori
Stomach
Epithelial Cells
Cell Survival
Helicobacter
Neutral Red
Virulence
Urea
Cultured Cells
Adenocarcinoma
Coloring Agents
Cell Line
Enzymes
acetohydroxamic acid
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Smoot, D. T., Mobley, H. L. T., Chippendale, G. R., Lewison, J. F., & Resau, J. H. (1990). Helicobacter pylori urease activity is toxic to human gastric epithelial cells. Infection and Immunity, 58(6), 1992-1994.

Helicobacter pylori urease activity is toxic to human gastric epithelial cells. / Smoot, D. T.; Mobley, H. L T; Chippendale, G. R.; Lewison, J. F.; Resau, J. H.

In: Infection and Immunity, Vol. 58, No. 6, 1990, p. 1992-1994.

Research output: Contribution to journalArticle

Smoot, DT, Mobley, HLT, Chippendale, GR, Lewison, JF & Resau, JH 1990, 'Helicobacter pylori urease activity is toxic to human gastric epithelial cells', Infection and Immunity, vol. 58, no. 6, pp. 1992-1994.
Smoot DT, Mobley HLT, Chippendale GR, Lewison JF, Resau JH. Helicobacter pylori urease activity is toxic to human gastric epithelial cells. Infection and Immunity. 1990;58(6):1992-1994.
Smoot, D. T. ; Mobley, H. L T ; Chippendale, G. R. ; Lewison, J. F. ; Resau, J. H. / Helicobacter pylori urease activity is toxic to human gastric epithelial cells. In: Infection and Immunity. 1990 ; Vol. 58, No. 6. pp. 1992-1994.
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