Helicobacter pylori IgG antibodies in aqueous humor and serum of subjects with primary open angle and pseudo-exfoliation glaucoma in a South Indian population

Nitin Deshpande, Prajna Lalitha, S. R. Krishna Das, Jitendra Jethani, R. Manju Pillai, Alan L. Robin, Karthik

Research output: Contribution to journalArticle

Abstract

PURPOSE: A prospective, nonrandomized, comparative study was carried out to investigate levels of anti-Helicobacter pylori-specific IgG antibodies in the aqueous humor and serum of patients with primary open angle glaucoma (POAG), exfoliation syndrome [pseudo-exfoliation glaucoma (PXFG)], and with normotensive cataract patients, who served as controls. METHODS: Aqueous humor was aspirated at the beginning of glaucoma surgery from 50 eyes of 50 patients with POAG, with PXFG and at the beginning of phacoemulsification cataract surgery from controls. Serum samples were obtained. Anti-H. pylori IgG concentration in the aqueous humor and serum was measured by means of enzyme linked immunosorbent assay. RESULTS: Serum analysis of anti-H. pylori IgG antibodies revealed statistically significant difference between POAG and PXFG (52.26±52.51 vs. 25.22±35.27, P=0.01). Also, the difference between POAG and controls was statistically significant (54.05±55.04 vs. 33.83±41.73, P=0.04). However, on comparing PXFG with the control group, the difference was statistically insignificant (P=0.12). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in POAG and controls were not statistically different (3.93±5.14 vs. 2.65±2.87, respectively, P=0.73). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in PXFG and controls were not statistically different (8.87±30.25 vs. 2.65±2.87, respectively, P=0.83). There was also no statistical difference of IgG levels between POAG and PXFG (3.93±5.14 vs. 8.87±30.25, respectively, P=0.87). CONCLUSIONS: The levels of anti-H. pylori IgG titers in sera of individuals with POAG were significantly higher compared with PXFG and control groups. We support the hypothesis of the role of anti-H. pylori antibodies in causative mechanism for POAG. We could not find a significant link between the anti-H. pylori IgG antibodies and the PXFG.

Original languageEnglish (US)
Pages (from-to)605-610
Number of pages6
JournalJournal of Glaucoma
Volume17
Issue number8
DOIs
StatePublished - Dec 2008

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Exfoliation Syndrome
Aqueous Humor
Helicobacter pylori
Immunoglobulin G
Antibodies
Serum
Population
Cataract
Control Groups
Phacoemulsification
Primary Open Angle Glaucoma
Glaucoma
Enzyme-Linked Immunosorbent Assay

Keywords

  • Helicobacter pylori
  • Primary open angle glaucoma
  • Pseudo-exfoliation glaucoma

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Helicobacter pylori IgG antibodies in aqueous humor and serum of subjects with primary open angle and pseudo-exfoliation glaucoma in a South Indian population. / Deshpande, Nitin; Lalitha, Prajna; Krishna Das, S. R.; Jethani, Jitendra; Pillai, R. Manju; Robin, Alan L.; Karthik.

In: Journal of Glaucoma, Vol. 17, No. 8, 12.2008, p. 605-610.

Research output: Contribution to journalArticle

Deshpande, Nitin ; Lalitha, Prajna ; Krishna Das, S. R. ; Jethani, Jitendra ; Pillai, R. Manju ; Robin, Alan L. ; Karthik. / Helicobacter pylori IgG antibodies in aqueous humor and serum of subjects with primary open angle and pseudo-exfoliation glaucoma in a South Indian population. In: Journal of Glaucoma. 2008 ; Vol. 17, No. 8. pp. 605-610.
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T1 - Helicobacter pylori IgG antibodies in aqueous humor and serum of subjects with primary open angle and pseudo-exfoliation glaucoma in a South Indian population

AU - Deshpande, Nitin

AU - Lalitha, Prajna

AU - Krishna Das, S. R.

AU - Jethani, Jitendra

AU - Pillai, R. Manju

AU - Robin, Alan L.

AU - Karthik,

PY - 2008/12

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N2 - PURPOSE: A prospective, nonrandomized, comparative study was carried out to investigate levels of anti-Helicobacter pylori-specific IgG antibodies in the aqueous humor and serum of patients with primary open angle glaucoma (POAG), exfoliation syndrome [pseudo-exfoliation glaucoma (PXFG)], and with normotensive cataract patients, who served as controls. METHODS: Aqueous humor was aspirated at the beginning of glaucoma surgery from 50 eyes of 50 patients with POAG, with PXFG and at the beginning of phacoemulsification cataract surgery from controls. Serum samples were obtained. Anti-H. pylori IgG concentration in the aqueous humor and serum was measured by means of enzyme linked immunosorbent assay. RESULTS: Serum analysis of anti-H. pylori IgG antibodies revealed statistically significant difference between POAG and PXFG (52.26±52.51 vs. 25.22±35.27, P=0.01). Also, the difference between POAG and controls was statistically significant (54.05±55.04 vs. 33.83±41.73, P=0.04). However, on comparing PXFG with the control group, the difference was statistically insignificant (P=0.12). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in POAG and controls were not statistically different (3.93±5.14 vs. 2.65±2.87, respectively, P=0.73). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in PXFG and controls were not statistically different (8.87±30.25 vs. 2.65±2.87, respectively, P=0.83). There was also no statistical difference of IgG levels between POAG and PXFG (3.93±5.14 vs. 8.87±30.25, respectively, P=0.87). CONCLUSIONS: The levels of anti-H. pylori IgG titers in sera of individuals with POAG were significantly higher compared with PXFG and control groups. We support the hypothesis of the role of anti-H. pylori antibodies in causative mechanism for POAG. We could not find a significant link between the anti-H. pylori IgG antibodies and the PXFG.

AB - PURPOSE: A prospective, nonrandomized, comparative study was carried out to investigate levels of anti-Helicobacter pylori-specific IgG antibodies in the aqueous humor and serum of patients with primary open angle glaucoma (POAG), exfoliation syndrome [pseudo-exfoliation glaucoma (PXFG)], and with normotensive cataract patients, who served as controls. METHODS: Aqueous humor was aspirated at the beginning of glaucoma surgery from 50 eyes of 50 patients with POAG, with PXFG and at the beginning of phacoemulsification cataract surgery from controls. Serum samples were obtained. Anti-H. pylori IgG concentration in the aqueous humor and serum was measured by means of enzyme linked immunosorbent assay. RESULTS: Serum analysis of anti-H. pylori IgG antibodies revealed statistically significant difference between POAG and PXFG (52.26±52.51 vs. 25.22±35.27, P=0.01). Also, the difference between POAG and controls was statistically significant (54.05±55.04 vs. 33.83±41.73, P=0.04). However, on comparing PXFG with the control group, the difference was statistically insignificant (P=0.12). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in POAG and controls were not statistically different (3.93±5.14 vs. 2.65±2.87, respectively, P=0.73). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in PXFG and controls were not statistically different (8.87±30.25 vs. 2.65±2.87, respectively, P=0.83). There was also no statistical difference of IgG levels between POAG and PXFG (3.93±5.14 vs. 8.87±30.25, respectively, P=0.87). CONCLUSIONS: The levels of anti-H. pylori IgG titers in sera of individuals with POAG were significantly higher compared with PXFG and control groups. We support the hypothesis of the role of anti-H. pylori antibodies in causative mechanism for POAG. We could not find a significant link between the anti-H. pylori IgG antibodies and the PXFG.

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