HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand

P. Echeverria, D. N. Taylor, K. A. Bettelheim, A. Chatkaeomorakot, S. Changchawalit, A. Thongcharoen, U. Leksomboon

Research output: Contribution to journalArticle

Abstract

Enteropathogenic Escherichia coli (EPEC) was isolated from 11% of 148 Hmong children under 1 year old with diarrhea at a refugee camp in northern Thailand. Of 16 children with EPEC-associated diarrhea, 11 were infected with EPEC that adhered to HeLa cells in a diffuse pattern, 3 were infected with EPEC that adhered to HeLa cells in a localized adherence (LA) pattern, and 2 were infected with EPEC that were nonadherent. In Bangkok, EPEC was isolated from 6% of 64 children under 1 year old with diarrhea and 7% of 56 children of the same age without diarrhea. Of four children with diarrhea, two were infected with EPEC with an LA pattern, and two were infected with nonadherent EPEC. Of four children without diarrhea, one was infected with EPEC with an LA pattern, one was infected with EPEC that adhered in a diffuse pattern, and two were infected with nonadherent EPEC. The 21 EPEC isolates with an LA pattern hybridized with the EPEC adherence factor DNA probe. EPEC was the only enteric pathogen identified in 16 (80%) of 20 children with EPEC-associated diarrhea. EPEC was as frequently isolated from children under 1 year old as were other bacterial enteric pathogens. The problem of identifying EPEC with pools of polyvalent antisera are described, and the need to identify additional enteropathogenic determinants of EPEC is discussed.

Original languageEnglish (US)
Pages (from-to)1472-1475
Number of pages4
JournalJournal of Clinical Microbiology
Volume25
Issue number8
StatePublished - 1987
Externally publishedYes

Fingerprint

Enteropathogenic Escherichia coli
Thailand
HeLa Cells
Diarrhea
Refugees

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Echeverria, P., Taylor, D. N., Bettelheim, K. A., Chatkaeomorakot, A., Changchawalit, S., Thongcharoen, A., & Leksomboon, U. (1987). HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand. Journal of Clinical Microbiology, 25(8), 1472-1475.

HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand. / Echeverria, P.; Taylor, D. N.; Bettelheim, K. A.; Chatkaeomorakot, A.; Changchawalit, S.; Thongcharoen, A.; Leksomboon, U.

In: Journal of Clinical Microbiology, Vol. 25, No. 8, 1987, p. 1472-1475.

Research output: Contribution to journalArticle

Echeverria, P, Taylor, DN, Bettelheim, KA, Chatkaeomorakot, A, Changchawalit, S, Thongcharoen, A & Leksomboon, U 1987, 'HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand', Journal of Clinical Microbiology, vol. 25, no. 8, pp. 1472-1475.
Echeverria P, Taylor DN, Bettelheim KA, Chatkaeomorakot A, Changchawalit S, Thongcharoen A et al. HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand. Journal of Clinical Microbiology. 1987;25(8):1472-1475.
Echeverria, P. ; Taylor, D. N. ; Bettelheim, K. A. ; Chatkaeomorakot, A. ; Changchawalit, S. ; Thongcharoen, A. ; Leksomboon, U. / HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand. In: Journal of Clinical Microbiology. 1987 ; Vol. 25, No. 8. pp. 1472-1475.
@article{4bfc3950b8f34e0686bbd9e8845ef6df,
title = "HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand",
abstract = "Enteropathogenic Escherichia coli (EPEC) was isolated from 11{\%} of 148 Hmong children under 1 year old with diarrhea at a refugee camp in northern Thailand. Of 16 children with EPEC-associated diarrhea, 11 were infected with EPEC that adhered to HeLa cells in a diffuse pattern, 3 were infected with EPEC that adhered to HeLa cells in a localized adherence (LA) pattern, and 2 were infected with EPEC that were nonadherent. In Bangkok, EPEC was isolated from 6{\%} of 64 children under 1 year old with diarrhea and 7{\%} of 56 children of the same age without diarrhea. Of four children with diarrhea, two were infected with EPEC with an LA pattern, and two were infected with nonadherent EPEC. Of four children without diarrhea, one was infected with EPEC with an LA pattern, one was infected with EPEC that adhered in a diffuse pattern, and two were infected with nonadherent EPEC. The 21 EPEC isolates with an LA pattern hybridized with the EPEC adherence factor DNA probe. EPEC was the only enteric pathogen identified in 16 (80{\%}) of 20 children with EPEC-associated diarrhea. EPEC was as frequently isolated from children under 1 year old as were other bacterial enteric pathogens. The problem of identifying EPEC with pools of polyvalent antisera are described, and the need to identify additional enteropathogenic determinants of EPEC is discussed.",
author = "P. Echeverria and Taylor, {D. N.} and Bettelheim, {K. A.} and A. Chatkaeomorakot and S. Changchawalit and A. Thongcharoen and U. Leksomboon",
year = "1987",
language = "English (US)",
volume = "25",
pages = "1472--1475",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - HeLa cell-adherent enteropathogenic Escherichia coli in children under 1 year of age in Thailand

AU - Echeverria, P.

AU - Taylor, D. N.

AU - Bettelheim, K. A.

AU - Chatkaeomorakot, A.

AU - Changchawalit, S.

AU - Thongcharoen, A.

AU - Leksomboon, U.

PY - 1987

Y1 - 1987

N2 - Enteropathogenic Escherichia coli (EPEC) was isolated from 11% of 148 Hmong children under 1 year old with diarrhea at a refugee camp in northern Thailand. Of 16 children with EPEC-associated diarrhea, 11 were infected with EPEC that adhered to HeLa cells in a diffuse pattern, 3 were infected with EPEC that adhered to HeLa cells in a localized adherence (LA) pattern, and 2 were infected with EPEC that were nonadherent. In Bangkok, EPEC was isolated from 6% of 64 children under 1 year old with diarrhea and 7% of 56 children of the same age without diarrhea. Of four children with diarrhea, two were infected with EPEC with an LA pattern, and two were infected with nonadherent EPEC. Of four children without diarrhea, one was infected with EPEC with an LA pattern, one was infected with EPEC that adhered in a diffuse pattern, and two were infected with nonadherent EPEC. The 21 EPEC isolates with an LA pattern hybridized with the EPEC adherence factor DNA probe. EPEC was the only enteric pathogen identified in 16 (80%) of 20 children with EPEC-associated diarrhea. EPEC was as frequently isolated from children under 1 year old as were other bacterial enteric pathogens. The problem of identifying EPEC with pools of polyvalent antisera are described, and the need to identify additional enteropathogenic determinants of EPEC is discussed.

AB - Enteropathogenic Escherichia coli (EPEC) was isolated from 11% of 148 Hmong children under 1 year old with diarrhea at a refugee camp in northern Thailand. Of 16 children with EPEC-associated diarrhea, 11 were infected with EPEC that adhered to HeLa cells in a diffuse pattern, 3 were infected with EPEC that adhered to HeLa cells in a localized adherence (LA) pattern, and 2 were infected with EPEC that were nonadherent. In Bangkok, EPEC was isolated from 6% of 64 children under 1 year old with diarrhea and 7% of 56 children of the same age without diarrhea. Of four children with diarrhea, two were infected with EPEC with an LA pattern, and two were infected with nonadherent EPEC. Of four children without diarrhea, one was infected with EPEC with an LA pattern, one was infected with EPEC that adhered in a diffuse pattern, and two were infected with nonadherent EPEC. The 21 EPEC isolates with an LA pattern hybridized with the EPEC adherence factor DNA probe. EPEC was the only enteric pathogen identified in 16 (80%) of 20 children with EPEC-associated diarrhea. EPEC was as frequently isolated from children under 1 year old as were other bacterial enteric pathogens. The problem of identifying EPEC with pools of polyvalent antisera are described, and the need to identify additional enteropathogenic determinants of EPEC is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0023270598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023270598&partnerID=8YFLogxK

M3 - Article

C2 - 3305563

AN - SCOPUS:0023270598

VL - 25

SP - 1472

EP - 1475

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 8

ER -