HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand

P. Echeverria, D. N. Taylor, A. Donohue-Rolfe, K. Supawat, O. Ratchtrachenchai, J. Kaper, G. T. Keusch

Research output: Contribution to journalArticle

Abstract

Enteropathogenic Escherichia coli (EPEC) strains isolated from hospitalized infants with diarrhea in Thailand were examined for HeLa cell adherence and cytotoxin production. Of 101 strains examined, 56 adhered to HeLa cells in a localized pattern (LA), 27 adhered in a diffuse pattern (DA), and 18 did not adhere. All 56 LA EPEC strains were O:K serotype O119:K69. A total of 20 (83%) of 24 EPEC O86:K61 strains and 7 (38%) of 19 EPEC strains belonging to six other O:K serotypes exhibited DA. All LA EPEC strains hybridized with a DNA probe for genes encoding EPEC adherence factor, whereas none of the 27 DA or 18 nonadherent EPEC strains hybridized with EPEC adherence factor probe. Sonic extracts of 57 (58%) of 98 EPEC strains tested at a dilution of 1:100 caused greater than 25% mortality of HeLa cell monolayers. A total of 50 (88%) of 57 cytotoxic sonic extracts were inhibited to various degrees of 1:500 dilution of polyclonal rabbit antisera to purified Shiga toxin. The mean percent inhibition of cytotoxic sonic extracts by anti-Shiga toxin was 67% (range, 29 to 89%). Fifty percent (38 of 56) of LA EPEC strains, fifty-two percent (14 of 27) of DA EPEC strains, and fifty-three percent (8 of 15) of nonadherent EPEC strains produced Shiga-like toxins. Both adherence and low levels of cell-associated cytotoxins were identified in EPEC strains from Thailand, but there did not appear to be an association between these two factors.

Original languageEnglish (US)
Pages (from-to)1519-1523
Number of pages5
JournalJournal of Clinical Microbiology
Volume25
Issue number8
StatePublished - 1987
Externally publishedYes

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Enteropathogenic Escherichia coli
Cytotoxins
Thailand
HeLa Cells
Diarrhea
Shiga Toxin
Shiga Toxins
DNA Probes
Immune Sera

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Echeverria, P., Taylor, D. N., Donohue-Rolfe, A., Supawat, K., Ratchtrachenchai, O., Kaper, J., & Keusch, G. T. (1987). HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand. Journal of Clinical Microbiology, 25(8), 1519-1523.

HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand. / Echeverria, P.; Taylor, D. N.; Donohue-Rolfe, A.; Supawat, K.; Ratchtrachenchai, O.; Kaper, J.; Keusch, G. T.

In: Journal of Clinical Microbiology, Vol. 25, No. 8, 1987, p. 1519-1523.

Research output: Contribution to journalArticle

Echeverria, P, Taylor, DN, Donohue-Rolfe, A, Supawat, K, Ratchtrachenchai, O, Kaper, J & Keusch, GT 1987, 'HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand', Journal of Clinical Microbiology, vol. 25, no. 8, pp. 1519-1523.
Echeverria P, Taylor DN, Donohue-Rolfe A, Supawat K, Ratchtrachenchai O, Kaper J et al. HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand. Journal of Clinical Microbiology. 1987;25(8):1519-1523.
Echeverria, P. ; Taylor, D. N. ; Donohue-Rolfe, A. ; Supawat, K. ; Ratchtrachenchai, O. ; Kaper, J. ; Keusch, G. T. / HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand. In: Journal of Clinical Microbiology. 1987 ; Vol. 25, No. 8. pp. 1519-1523.
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abstract = "Enteropathogenic Escherichia coli (EPEC) strains isolated from hospitalized infants with diarrhea in Thailand were examined for HeLa cell adherence and cytotoxin production. Of 101 strains examined, 56 adhered to HeLa cells in a localized pattern (LA), 27 adhered in a diffuse pattern (DA), and 18 did not adhere. All 56 LA EPEC strains were O:K serotype O119:K69. A total of 20 (83{\%}) of 24 EPEC O86:K61 strains and 7 (38{\%}) of 19 EPEC strains belonging to six other O:K serotypes exhibited DA. All LA EPEC strains hybridized with a DNA probe for genes encoding EPEC adherence factor, whereas none of the 27 DA or 18 nonadherent EPEC strains hybridized with EPEC adherence factor probe. Sonic extracts of 57 (58{\%}) of 98 EPEC strains tested at a dilution of 1:100 caused greater than 25{\%} mortality of HeLa cell monolayers. A total of 50 (88{\%}) of 57 cytotoxic sonic extracts were inhibited to various degrees of 1:500 dilution of polyclonal rabbit antisera to purified Shiga toxin. The mean percent inhibition of cytotoxic sonic extracts by anti-Shiga toxin was 67{\%} (range, 29 to 89{\%}). Fifty percent (38 of 56) of LA EPEC strains, fifty-two percent (14 of 27) of DA EPEC strains, and fifty-three percent (8 of 15) of nonadherent EPEC strains produced Shiga-like toxins. Both adherence and low levels of cell-associated cytotoxins were identified in EPEC strains from Thailand, but there did not appear to be an association between these two factors.",
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AU - Echeverria, P.

AU - Taylor, D. N.

AU - Donohue-Rolfe, A.

AU - Supawat, K.

AU - Ratchtrachenchai, O.

AU - Kaper, J.

AU - Keusch, G. T.

PY - 1987

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N2 - Enteropathogenic Escherichia coli (EPEC) strains isolated from hospitalized infants with diarrhea in Thailand were examined for HeLa cell adherence and cytotoxin production. Of 101 strains examined, 56 adhered to HeLa cells in a localized pattern (LA), 27 adhered in a diffuse pattern (DA), and 18 did not adhere. All 56 LA EPEC strains were O:K serotype O119:K69. A total of 20 (83%) of 24 EPEC O86:K61 strains and 7 (38%) of 19 EPEC strains belonging to six other O:K serotypes exhibited DA. All LA EPEC strains hybridized with a DNA probe for genes encoding EPEC adherence factor, whereas none of the 27 DA or 18 nonadherent EPEC strains hybridized with EPEC adherence factor probe. Sonic extracts of 57 (58%) of 98 EPEC strains tested at a dilution of 1:100 caused greater than 25% mortality of HeLa cell monolayers. A total of 50 (88%) of 57 cytotoxic sonic extracts were inhibited to various degrees of 1:500 dilution of polyclonal rabbit antisera to purified Shiga toxin. The mean percent inhibition of cytotoxic sonic extracts by anti-Shiga toxin was 67% (range, 29 to 89%). Fifty percent (38 of 56) of LA EPEC strains, fifty-two percent (14 of 27) of DA EPEC strains, and fifty-three percent (8 of 15) of nonadherent EPEC strains produced Shiga-like toxins. Both adherence and low levels of cell-associated cytotoxins were identified in EPEC strains from Thailand, but there did not appear to be an association between these two factors.

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