Abstract
We studied the H-chain class switch rearrangement in four groups of clonally related B cell hybridomas, to test the hypothesis that class switch terminates somatic mutation in a B cell. Using switch region-specific probes in Southern blot analysis individual μ-γ switch rearrangement events can be distinguished. We show that clonally related IgG-producing hybridomas that differ by mutations often share a common switch rearrangement. This indicates that class switch in these cells did not terminate somatic mutation.
Original language | English (US) |
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Pages (from-to) | 531-536 |
Number of pages | 6 |
Journal | Journal of Experimental Medicine |
Volume | 172 |
Issue number | 2 |
State | Published - Aug 1 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology