Heavy chain binding protein recognizes incompletely disulfidebonded forms of vesicular stomatitis virus G protein

Carolyn E Machamer, Robert W. Doms, David G. Bolel, Ari Helenius, John K. Rose

Research output: Contribution to journalArticle

Abstract

To investigate the function of heavy chain binding protein (BiP, GRP 78) in the endoplasmic reticulum, we have characterized its interaction with a model plasma membrane glycoprotein, the G protein of vesicular stomatitis virus. We used a panel of well characterized mutant G proteins and immunoprecipitation with anti-BiP antibodies to determine if BiP interacted with newly synthesized G protein and/or mutant G proteins retained in the endoplasmic reticulum. We made three major observations: 1) BiP bound transiently to folding intermediates of wild-type G protein which were incompletely disulfide-bonded; 2) BiP did not bind stably to all mutant G proteins which remain in the endoplasmic reticulum; and 3) BiP bound stably only to mutant G proteins which do not form correct intrachain disulfide bonds.

Original languageEnglish (US)
Pages (from-to)6879-6883
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number12
StatePublished - 1990

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GTP-Binding Proteins
Carrier Proteins
Mutant Proteins
Endoplasmic Reticulum
Disulfides
Membrane Glycoproteins
Cell membranes
Immunoprecipitation
vesicular stomatitis virus G protein
Anti-Idiotypic Antibodies
Cell Membrane
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Heavy chain binding protein recognizes incompletely disulfidebonded forms of vesicular stomatitis virus G protein. / Machamer, Carolyn E; Doms, Robert W.; Bolel, David G.; Helenius, Ari; Rose, John K.

In: Journal of Biological Chemistry, Vol. 265, No. 12, 1990, p. 6879-6883.

Research output: Contribution to journalArticle

Machamer, Carolyn E ; Doms, Robert W. ; Bolel, David G. ; Helenius, Ari ; Rose, John K. / Heavy chain binding protein recognizes incompletely disulfidebonded forms of vesicular stomatitis virus G protein. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 12. pp. 6879-6883.
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