Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein

C. E. Machamer, R. W. Doms, D. G. Bole, A. Helenius, J. K. Rose

Research output: Contribution to journalArticle

Abstract

To investigate the function of heavy chain binding protein (BiP, GRP 78) in the endoplasmic reticulum, we have characterized its interaction with a model plasma membrane glycoprotein, the G protein of vesicular stomatitis virus. We used a panel of well characterized mutant G proteins and immunoprecipitation with anti-BiP antibodies to determine if BiP interacted with newly synthesized G protein and/or mutant G proteins retained in the endoplasmic reticulum. We made three major observations: 1) BiP bound transiently to folding intermediates of wild-type G protein which were incompletely disulfide-bonded; 2) BiP did not bind stably to all mutant G proteins which remain in the endoplasmic reticulum; and 3) BiP bound stably only to mutant G proteins which do not form correct intrachain disulfide bonds.

Original languageEnglish (US)
Pages (from-to)6879-6883
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number12
StatePublished - May 16 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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