Heating induces aggregation and decreases detection of serotonin transporter protein on western blots

Michael W. McLane, George Hatzidimitriou, Jie Yuan, Una McCann, George Ricaurte

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, there has been growing interest in the use of Western blot analysis to monitor changes in the abundance of the serotonin transporter (SERT) protein. In the Western blot procedure, heat denaturation is a common, early step. We now report that heating samples to 90°C decreases the abundance of the SERT protein band and causes dispersion of a majority of the SERT signal to a high molecular weight smear. These observations are in keeping with the fact that heating can influence the electrophoretic behavior of some proteins. By omitting the heat denaturation step in the Western blot procedure, better detection of the SERT protein is achieved.

Original languageEnglish (US)
Pages (from-to)875-876
Number of pages2
JournalSynapse
Volume61
Issue number10
DOIs
StatePublished - Oct 2007

Keywords

  • Electrophoresis
  • MDMA
  • Serotonin transporter
  • Thermal aggregation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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