Heat shock proteins can regulate expression of the Tla region-encoded class Ib molecule Qa-1

Farhad Imani, Mark J. Soloski

Research output: Contribution to journalArticlepeer-review

Abstract

T cells recognize foreign antigens in association with the highly polymorphic class I and class II molecules encoded in the major histocompatibility complex (MHC). In addition to these highly polymorphic molecules, the murine MHC also encodes, in the Qa/Tla region, several less polymorphic structures referred to as class 1-like or class Ib molecules. Although no specific function has been assigned to these molecules, their overall structural similarities to the classical class I molecules and their association with β2-microglobulin suggest a role in antigen recognition. Recent data have suggested that the class Ib molecule Qa-1 may be involved in antigen presentation to T cells expressing γδ receptors. In addition, several reports have demonstrated that γδ T cells can respond to mycobacterial heat shock proteins. We report that transfection of a mouse fibroblast line with gene T23b leads to the surface expression of a molecule that is structurally identical to lymphocyte Qa-1b. In the transfected cells the predominant Qa-1 species was present in an immature intracellular form. The expression of mature cell surface Qa-1 was dramatically and selectively increased following heat shock. Furthermore, the addition of a tryptic digest of Mycobacterium bovis 65-kDa heat shock protein stabilized the surface expression of Qa-1b. These observations suggest that the Qa-1 molecule may be involved in the presentation of heat shock protein-derived peptides to the immune system.

Original languageEnglish (US)
Pages (from-to)10475-10479
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number23
DOIs
StatePublished - 1991

Keywords

  • Antigen presentation
  • Major histocompatibility complex

ASJC Scopus subject areas

  • General

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