Heat-shock-induced enhanced reactivation of UV-irradiated Herpesvirus

James D Yager, Joanne Zurlo, Arthur L. Penn

Research output: Contribution to journalArticle

Abstract

The objective of this study was to compare the ability of heat stock (HS) with that of another type of cellular stress, UV irradiation, to cause the induction of enhanced viral reactivation, a process that may represent an SOS-type repair process in mammalian cells. Studies performed to evaluate the effect of HS on growth of Vero cells revealed that HS at 45°C for 45 min caused inhibition of cell growth similar to that caused by UV irradiation at 12 J/m2, but this inhibition was not observed at HS treatment for 5-15 min, or at a UV fluence of 2 J/m2. Enhanced reactivation of UV-irradiated Herpesvierus was observed in cells which had been pretreated by HS for > 30 min or UV at 12 J/m2. The synthesis of new proteins following HS for 15 and 45 min and UV at 12 J/m2 was examined by [35S]methionine-labeling experiments. The new synthesis of two HS proteins with molecular weights of 46 000 and 78 000 was induced by both levels of HS, but to a much greater extent at the high dose. These proteins were not detected in response to UV irradiation. These results indicate that, like UV irradiation, HS at levels inhibitory to cell growth induced enhanced viral reactivation in Vero cells. The results also suggest that at least two proteins in the HS protein family are not necessary for this response to occur.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalMutation Research DNA Repair Reports
Volume146
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Herpesviridae
Shock
Hot Temperature
Vero Cells
Proteins
Growth
Methionine
Molecular Weight

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Heat-shock-induced enhanced reactivation of UV-irradiated Herpesvirus. / Yager, James D; Zurlo, Joanne; Penn, Arthur L.

In: Mutation Research DNA Repair Reports, Vol. 146, No. 2, 1985, p. 121-128.

Research output: Contribution to journalArticle

Yager, James D ; Zurlo, Joanne ; Penn, Arthur L. / Heat-shock-induced enhanced reactivation of UV-irradiated Herpesvirus. In: Mutation Research DNA Repair Reports. 1985 ; Vol. 146, No. 2. pp. 121-128.
@article{7a28956e367644269105c0595b3679f4,
title = "Heat-shock-induced enhanced reactivation of UV-irradiated Herpesvirus",
abstract = "The objective of this study was to compare the ability of heat stock (HS) with that of another type of cellular stress, UV irradiation, to cause the induction of enhanced viral reactivation, a process that may represent an SOS-type repair process in mammalian cells. Studies performed to evaluate the effect of HS on growth of Vero cells revealed that HS at 45°C for 45 min caused inhibition of cell growth similar to that caused by UV irradiation at 12 J/m2, but this inhibition was not observed at HS treatment for 5-15 min, or at a UV fluence of 2 J/m2. Enhanced reactivation of UV-irradiated Herpesvierus was observed in cells which had been pretreated by HS for > 30 min or UV at 12 J/m2. The synthesis of new proteins following HS for 15 and 45 min and UV at 12 J/m2 was examined by [35S]methionine-labeling experiments. The new synthesis of two HS proteins with molecular weights of 46 000 and 78 000 was induced by both levels of HS, but to a much greater extent at the high dose. These proteins were not detected in response to UV irradiation. These results indicate that, like UV irradiation, HS at levels inhibitory to cell growth induced enhanced viral reactivation in Vero cells. The results also suggest that at least two proteins in the HS protein family are not necessary for this response to occur.",
author = "Yager, {James D} and Joanne Zurlo and Penn, {Arthur L.}",
year = "1985",
doi = "10.1016/0167-8817(85)90001-X",
language = "English (US)",
volume = "146",
pages = "121--128",
journal = "Mutation Research DNA Repair Reports",
issn = "0167-8817",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Heat-shock-induced enhanced reactivation of UV-irradiated Herpesvirus

AU - Yager, James D

AU - Zurlo, Joanne

AU - Penn, Arthur L.

PY - 1985

Y1 - 1985

N2 - The objective of this study was to compare the ability of heat stock (HS) with that of another type of cellular stress, UV irradiation, to cause the induction of enhanced viral reactivation, a process that may represent an SOS-type repair process in mammalian cells. Studies performed to evaluate the effect of HS on growth of Vero cells revealed that HS at 45°C for 45 min caused inhibition of cell growth similar to that caused by UV irradiation at 12 J/m2, but this inhibition was not observed at HS treatment for 5-15 min, or at a UV fluence of 2 J/m2. Enhanced reactivation of UV-irradiated Herpesvierus was observed in cells which had been pretreated by HS for > 30 min or UV at 12 J/m2. The synthesis of new proteins following HS for 15 and 45 min and UV at 12 J/m2 was examined by [35S]methionine-labeling experiments. The new synthesis of two HS proteins with molecular weights of 46 000 and 78 000 was induced by both levels of HS, but to a much greater extent at the high dose. These proteins were not detected in response to UV irradiation. These results indicate that, like UV irradiation, HS at levels inhibitory to cell growth induced enhanced viral reactivation in Vero cells. The results also suggest that at least two proteins in the HS protein family are not necessary for this response to occur.

AB - The objective of this study was to compare the ability of heat stock (HS) with that of another type of cellular stress, UV irradiation, to cause the induction of enhanced viral reactivation, a process that may represent an SOS-type repair process in mammalian cells. Studies performed to evaluate the effect of HS on growth of Vero cells revealed that HS at 45°C for 45 min caused inhibition of cell growth similar to that caused by UV irradiation at 12 J/m2, but this inhibition was not observed at HS treatment for 5-15 min, or at a UV fluence of 2 J/m2. Enhanced reactivation of UV-irradiated Herpesvierus was observed in cells which had been pretreated by HS for > 30 min or UV at 12 J/m2. The synthesis of new proteins following HS for 15 and 45 min and UV at 12 J/m2 was examined by [35S]methionine-labeling experiments. The new synthesis of two HS proteins with molecular weights of 46 000 and 78 000 was induced by both levels of HS, but to a much greater extent at the high dose. These proteins were not detected in response to UV irradiation. These results indicate that, like UV irradiation, HS at levels inhibitory to cell growth induced enhanced viral reactivation in Vero cells. The results also suggest that at least two proteins in the HS protein family are not necessary for this response to occur.

UR - http://www.scopus.com/inward/record.url?scp=0022390664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022390664&partnerID=8YFLogxK

U2 - 10.1016/0167-8817(85)90001-X

DO - 10.1016/0167-8817(85)90001-X

M3 - Article

VL - 146

SP - 121

EP - 128

JO - Mutation Research DNA Repair Reports

JF - Mutation Research DNA Repair Reports

SN - 0167-8817

IS - 2

ER -