HBXAP, a novel PHD-finger protein, possesses transcription repression activity

Meir Shamay, Orr Barak, Yosef Shaul

Research output: Contribution to journalArticle

Abstract

The PHD/LAP (plant homology domain/leukemia associated protein) finger motif is characteristically defined by a histidine and seven cysteines that are spatially arranged in a C4HC3 consensus sequence. This unique zinc finger, found primarily in a wide variety of chromatin-associated proteins, is considered to mediate protein-protein interactions. We have isolated a novel human PHD-finger protein, HBXAP (for hepatitis B virus x associated protein). HBXAP has three alternatively spliced isoforms. We also identified the Drosophila melanogaster HBXAP ortholog, gene CG8677. Based on alignment of four different proteins, we found a novel conserved domain in HBXAP that we designated the HBXAP conserved domain (XCD). We show that HBXAP represses transcription when recruited to DNA via the DNA binding of GAL4. Furthermore, the PHD finger alone suffices to repress transcription, thus attributing a functional role to this domain. The gene HBXAP is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, suggesting a role for HBXAP in tumorigenesis similar to that of other PHD-containing proteins.

Original languageEnglish (US)
Pages (from-to)523-529
Number of pages7
JournalGenomics
Volume79
Issue number4
DOIs
StatePublished - Jan 1 2002

Keywords

  • Breast cancer
  • Chromosome 11q13
  • Gene amplification
  • HBV
  • HBXAP
  • HBx
  • HCC
  • PHD finger
  • XCD
  • pX

ASJC Scopus subject areas

  • Genetics

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