@inbook{59066a885dff4d208655cc3d72f96711,
title = "Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein",
abstract = "We recently developed a novel expression system employing the halophilic Archaeon Halobacterium sp. NRC-1 for scaled-up production and nanoparticle-display of antigenic proteins. Here, we have targeted the major human parasite Plasmodium falciparum circumsporozoite protein (CSP), which is of interest for formulation of a protective malaria vaccine. A codon-optimized synthetic gene coding the full-length CSP was inserted downstream of the strong promoter for gvpA, the major gas vesicle nanoparticle protein gene, in the pDRK expression vector, and as a fusion to the gvpC protein in the pSD expression vector for display on the surface of gas vesicle nanoparticles. We found that the pDRK-CSP expression plasmid programmed high-level production of full-length CSP and the pSD-CSP expression plasmid programmed production of a GvpC-CSP fusion protein, for display on gas vesicle nanoparticles. The Halobacterium sp. expression system provides a novel approach and a potentially valuable technical advancement for the production of P. falciparum CSP for malaria vaccine development.",
keywords = "Falciparum Circumsporozoite Protein, Falciparum Sporozoite, Halobacterium Cell, Hypotonic Condition, Strain SD109",
author = "Pecher, {Wolf T.} and Kim, {Jong Myoung} and Priya DasSarma and Ram Karan and Photini Sinnis and Shiladitya DasSarma",
note = "Funding Information: OPP1061509, and National Institutes of Health grant R03 AI107634 to SD and National Institutes of Health grant R01 AI056840 to PS. JK was supported by the PKNU Research Abroad Fund CD-2013-0914. We thank Susan Barnes and Folasade Ekulona for technical assistance, Stefanie Trop and Peter Dumoulin for valuable discussions, and Prof. F. Zavala for critical reading of the manuscript. Funding Information: Acknowledgments This work was supported by Bill & Melinda Gates Foundation grant Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2016.",
year = "2016",
doi = "10.1007/978-3-319-13521-2_25",
language = "English (US)",
series = "Grand Challenges in Biology and Biotechnology",
publisher = "Springer Science and Business Media B.V.",
pages = "699--709",
booktitle = "Grand Challenges in Biology and Biotechnology",
}