Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein

Wolf T. Pecher, Jong Myoung Kim, Priya DasSarma, Ram Karan, Photini Sinnis, Shiladitya DasSarma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We recently developed a novel expression system employing the halophilic Archaeon Halobacterium sp. NRC-1 for scaled-up production and nanoparticle-display of antigenic proteins. Here, we have targeted the major human parasite Plasmodium falciparum circumsporozoite protein (CSP), which is of interest for formulation of a protective malaria vaccine. A codon-optimized synthetic gene coding the full-length CSP was inserted downstream of the strong promoter for gvpA, the major gas vesicle nanoparticle protein gene, in the pDRK expression vector, and as a fusion to the gvpC protein in the pSD expression vector for display on the surface of gas vesicle nanoparticles. We found that the pDRK-CSP expression plasmid programmed high-level production of full-length CSP and the pSD-CSP expression plasmid programmed production of a GvpC-CSP fusion protein, for display on gas vesicle nanoparticles. The Halobacterium sp. expression system provides a novel approach and a potentially valuable technical advancement for the production of P. falciparum CSP for malaria vaccine development.

Original languageEnglish (US)
Title of host publicationGrand Challenges in Biology and Biotechnology
PublisherSpringer Science and Business Media B.V.
Pages699-709
Number of pages11
DOIs
StatePublished - 2016

Publication series

NameGrand Challenges in Biology and Biotechnology
Volume1
ISSN (Print)2367-1017
ISSN (Electronic)2367-1025

Keywords

  • Falciparum Circumsporozoite Protein
  • Falciparum Sporozoite
  • Halobacterium Cell
  • Hypotonic Condition
  • Strain SD109

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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