Hair-cell mechanotransduction persists in TRP channel knockout mice

Xudong Wu, Artur A. Indzhykulian, Paul D. Niksch, Roxanna M. Webber, Miguel Garcia-Gonzalez, Terry Watnick, Jing Zhou, Melissa A. Vollrath, David P. Corey

Research output: Contribution to journalArticle

Abstract

Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore properties not similar to the hair-cell channel. Such studies have excluded Trpv4, Trpa1, Trpml3, Trpm1, Trpm3, Trpc1, Trpc3, Trpc5, and Trpc6. However, others remain reasonable candidates. We used data from an RNA-seq analysis of gene expression in hair cells as well as data on TRP channel conductance to narrow the candidate group. We then characterized mice lacking functional Trpm2, Pkd2, Pkd2l1, Pkd2l2 and Pkd1l3, using scanning electron microscopy, auditory brainstem response, permeant dye accumulation, and single-cell electrophysiology. In all of these TRP-deficient mice, and in double and triple knockouts, mechanotransduction persisted. Together with published studies, these results argue against the participation of any of the 33 mouse TRP channels in hair cell transduction.

Original languageEnglish (US)
Article numbere0155577
JournalPLoS One
Volume11
Issue number5
DOIs
StatePublished - May 1 2016
Externally publishedYes

Fingerprint

Knockout Mice
Hair
hairs
Cells
mice
Electrophysiology
cells
Ion Channels
Gene expression
Animals
Coloring Agents
electrophysiology
RNA
Brain Stem Auditory Evoked Potentials
ion channels
brain stem
Inner Ear
Scanning electron microscopy
Electron Scanning Microscopy
dyes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wu, X., Indzhykulian, A. A., Niksch, P. D., Webber, R. M., Garcia-Gonzalez, M., Watnick, T., ... Corey, D. P. (2016). Hair-cell mechanotransduction persists in TRP channel knockout mice. PLoS One, 11(5), [e0155577]. https://doi.org/10.1371/journal.pone.0155577

Hair-cell mechanotransduction persists in TRP channel knockout mice. / Wu, Xudong; Indzhykulian, Artur A.; Niksch, Paul D.; Webber, Roxanna M.; Garcia-Gonzalez, Miguel; Watnick, Terry; Zhou, Jing; Vollrath, Melissa A.; Corey, David P.

In: PLoS One, Vol. 11, No. 5, e0155577, 01.05.2016.

Research output: Contribution to journalArticle

Wu, X, Indzhykulian, AA, Niksch, PD, Webber, RM, Garcia-Gonzalez, M, Watnick, T, Zhou, J, Vollrath, MA & Corey, DP 2016, 'Hair-cell mechanotransduction persists in TRP channel knockout mice', PLoS One, vol. 11, no. 5, e0155577. https://doi.org/10.1371/journal.pone.0155577
Wu X, Indzhykulian AA, Niksch PD, Webber RM, Garcia-Gonzalez M, Watnick T et al. Hair-cell mechanotransduction persists in TRP channel knockout mice. PLoS One. 2016 May 1;11(5). e0155577. https://doi.org/10.1371/journal.pone.0155577
Wu, Xudong ; Indzhykulian, Artur A. ; Niksch, Paul D. ; Webber, Roxanna M. ; Garcia-Gonzalez, Miguel ; Watnick, Terry ; Zhou, Jing ; Vollrath, Melissa A. ; Corey, David P. / Hair-cell mechanotransduction persists in TRP channel knockout mice. In: PLoS One. 2016 ; Vol. 11, No. 5.
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