TY - JOUR
T1 - Haemophilus somnus activation of brain endothelial cells
T2 - Potential role for local cytokine production and thrombosis in central nervous system (CNS) infection
AU - Behling-Kelly, Erica
AU - Kim, Kwang S.
AU - Czuprynski, Charles J.
PY - 2007/10
Y1 - 2007/10
N2 - Thrombotic meningoencephalitis (TME) is a neurological condition in cattle characterized by fibrinopurulent meningitis with hemorrhage, abscess formation and thrombotic vasculitis throughout the central nervous system. The etiologic agent of TME is Haemophilus somnus, a gram-negative pleomorphic coccobacillus. Although the pathogenesis of TME is not well understood, the propensity of H. somnus to cause vasculitis and intravascular thrombosis suggests a critical role for the interactions between the bacteria and endothelial cells in inciting the disease. The goal of this study was to determine if H. somnus elicits an inflammatory and procoagulative response in bovine brain microvascular endothelial cells (BBEC) in vitro. We demonstrate that BBEC exposed to H. somnus secrete significant levels of the proinflammatory and procoagulative cytokines TNF-α and IL-1β. BBEC treated with H. somnus also display increased levels of IL-6 mRNA, another cytokine associated with coagulopathy in vivo. H. somnus-treated BBEC exhibited increased procoagulant activity and tissue factor expression and activity, along with a decreased ability to activate protein C and decreased expression of thrombomodulin mRNA. These changes would be expected to promote thrombus formation in vessels of the CNS, and potentially contribute to the pathogenesis of TME.
AB - Thrombotic meningoencephalitis (TME) is a neurological condition in cattle characterized by fibrinopurulent meningitis with hemorrhage, abscess formation and thrombotic vasculitis throughout the central nervous system. The etiologic agent of TME is Haemophilus somnus, a gram-negative pleomorphic coccobacillus. Although the pathogenesis of TME is not well understood, the propensity of H. somnus to cause vasculitis and intravascular thrombosis suggests a critical role for the interactions between the bacteria and endothelial cells in inciting the disease. The goal of this study was to determine if H. somnus elicits an inflammatory and procoagulative response in bovine brain microvascular endothelial cells (BBEC) in vitro. We demonstrate that BBEC exposed to H. somnus secrete significant levels of the proinflammatory and procoagulative cytokines TNF-α and IL-1β. BBEC treated with H. somnus also display increased levels of IL-6 mRNA, another cytokine associated with coagulopathy in vivo. H. somnus-treated BBEC exhibited increased procoagulant activity and tissue factor expression and activity, along with a decreased ability to activate protein C and decreased expression of thrombomodulin mRNA. These changes would be expected to promote thrombus formation in vessels of the CNS, and potentially contribute to the pathogenesis of TME.
KW - Brain
KW - Endothelial
KW - Haemophilus
KW - Infection
KW - Thrombosis
UR - http://www.scopus.com/inward/record.url?scp=35048891928&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35048891928&partnerID=8YFLogxK
U2 - 10.1160/TH06-11-0665
DO - 10.1160/TH06-11-0665
M3 - Article
C2 - 17938807
AN - SCOPUS:35048891928
SN - 0340-6245
VL - 98
SP - 823
EP - 830
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 4
ER -