TY - JOUR
T1 - H-2 expression on a teratocarcinoma-derived cell line, TerC
AU - Searls, D. B.
AU - Edidin, M.
PY - 1982
Y1 - 1982
N2 - The murine teratocarcinoma-derived cell line TerC, previously thought to lack products of the major histocompatibility locus H-2, was shown to express low levels of K- and D-end H-2 specificities with the use of a sensitive cytotoxic assay. The assay, based on the ability of antibody and complement to inhibit uptake of the thymidine analogue [125I]5-iodo-2'-deoxyuridine, detected appropriate public and private specificities, as demonstrated with the use of oligospecific antisera and by absorption analyses. A series of clone of TerC varied only slightly in H-2 expression, and there was no tendency for expression to increase with time in culture; thus the low levels of H-2 were not the result of a differentiating subpopulation.
AB - The murine teratocarcinoma-derived cell line TerC, previously thought to lack products of the major histocompatibility locus H-2, was shown to express low levels of K- and D-end H-2 specificities with the use of a sensitive cytotoxic assay. The assay, based on the ability of antibody and complement to inhibit uptake of the thymidine analogue [125I]5-iodo-2'-deoxyuridine, detected appropriate public and private specificities, as demonstrated with the use of oligospecific antisera and by absorption analyses. A series of clone of TerC varied only slightly in H-2 expression, and there was no tendency for expression to increase with time in culture; thus the low levels of H-2 were not the result of a differentiating subpopulation.
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M3 - Article
C2 - 6958907
AN - SCOPUS:0020444954
SN - 0027-8874
VL - 69
SP - 1311
EP - 1315
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -