GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB

Osigwe Esue, Denis Wirtz, Yiider Tseng

Research output: Contribution to journalArticle

Abstract

MreB, a major component of the recently discovered bacterial cytoskeleton, displays a structure homologous to its eukaryotic counterpart actin. Here, we study the assembly and mechanical properties of Thermotoga maritima MreB in the presence of different nucleotides in vitro. We found that GTP, not ADP or GDP, can mediate MreB assembly into filamentous structures as effectively as ATP. Upon MreB assembly, both GTP and ATP release the gamma phosphate at similar rates. Therefore, MreB is an equally effective ATPase and GTPase. Electron microscopy and quantitative rheology suggest that the morphologies and micromechanical properties of filamentous ATP-MreB and GTP-MreB are similar. In contrast, mammalian actin assembly is favored in the presence of ATP over GTP. These results indicate that, despite high structural homology of their monomers, T. maritima MreB and actin filaments display different assembly, morphology, micromechanics, and nucleotide-binding specificity. Furthermore, the biophysical properties of T. maritima MreB filaments, including high rigidity and propensity to form bundles, suggest a mechanism by which MreB helical structure may be involved in imposing a cylindrical architecture on rod-shaped bacterial cells.

Original languageEnglish (US)
Pages (from-to)968-976
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number3
DOIs
StatePublished - Feb 2006

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Bacterial Proteins
GTP Phosphohydrolases
Thermotoga maritima
Guanosine Triphosphate
Cytoskeleton
Adenosine Triphosphate
Actins
Nucleotides
Rheology
Actin Cytoskeleton
Adenosine Diphosphate
Adenosine Triphosphatases
Electron Microscopy
Phosphates

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB. / Esue, Osigwe; Wirtz, Denis; Tseng, Yiider.

In: Journal of Bacteriology, Vol. 188, No. 3, 02.2006, p. 968-976.

Research output: Contribution to journalArticle

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