G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors

M. A. Zeiger, J. A. Norton, A. H. Harken, M. Demeure

Research output: Contribution to journalArticle

Abstract

Background. The isolation of mRNA molecules that are either uniquely or more highly expressed by tumor and not normal tissue is a powerful tool in the study of cell regulation and growth. To this end we constructed a complementary DNA (cDNA) library from messenger RNA (mRNA) isolated from a human insulinoma and, by differential hybridization with cDNA from both normal pancreas and insulinoma, isolated clones more highly expressed by insulinoma. Methods. Total RNA was isolated from human insulinoma and normal pancreas and purified to mRNA by oligo (dT) column. An insulinoma cDNA library was constructed and screened with 32P-labeled cDNA from pancreas and insulinoma. Northern blots from insulinoma, pancreas, carcinomas, normal endocrine tissues, and endocrine tumors were then probed with the 32P- labeled inserts. Results. Two clones that consistently hybridized with 32P cDNA from insulinoma and not pancreas proved to represent mRNAs for insulin and the α subunit of the G(s) protein. There was a markedly higher expression (30-fold) of the gene for G(s)α in mRNA from insulinoma compared with normal pancreas by Northern blot analysis. We found G(s)α to be more highly expressed by a pheochromocytoma, a corticotropin-producing islet cell tumor of the pancreas, and a corticotropin-producing thymic carcinoid (up to 35-fold) compared with normal pancreas, whereas normal endocrine tissues, a parathyroid adenoma, thyroid follicular adenoma, gastrinoma, and several carcinomas showed no expression. Conclusions. This study showed that G(s)α is highly expressed in insulinoma and certain endocrine tumors. It is not expressed in several cancers or normal endocrine tissues. Others have implicated mutated G(s) proteins in the tumorigenesis of pituitary and thyroid tumors. G proteins are also known to mediate hormonal transmembrane signaling. Its overexpression in four of seven endocrine tumors tested suggests that it may have a role in the unregulated hormone secretion and/or a role in the tumorigenesis of differentiated endocrine tumors.

Original languageEnglish (US)
Pages (from-to)458-463
Number of pages6
JournalSurgery
Volume114
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Gs GTP-Binding Protein alpha Subunits
Insulinoma
Pancreas
Genes
Neoplasms
Complementary DNA
Messenger RNA
Gene Library
Northern Blotting
Adrenocorticotropic Hormone
Carcinogenesis
Clone Cells
Gastrinoma
Islet Cell Adenoma
Carcinoma
Parathyroid Neoplasms
Carcinoid Tumor
Pituitary Neoplasms
Pheochromocytoma
Thyroid Neoplasms

ASJC Scopus subject areas

  • Surgery

Cite this

Zeiger, M. A., Norton, J. A., Harken, A. H., & Demeure, M. (1993). G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors. Surgery, 114(2), 458-463.

G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors. / Zeiger, M. A.; Norton, J. A.; Harken, A. H.; Demeure, M.

In: Surgery, Vol. 114, No. 2, 1993, p. 458-463.

Research output: Contribution to journalArticle

Zeiger, MA, Norton, JA, Harken, AH & Demeure, M 1993, 'G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors', Surgery, vol. 114, no. 2, pp. 458-463.
Zeiger MA, Norton JA, Harken AH, Demeure M. G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors. Surgery. 1993;114(2):458-463.
Zeiger, M. A. ; Norton, J. A. ; Harken, A. H. ; Demeure, M. / G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors. In: Surgery. 1993 ; Vol. 114, No. 2. pp. 458-463.
@article{121f0ae1a1f24e20bb189c958772eeec,
title = "G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors",
abstract = "Background. The isolation of mRNA molecules that are either uniquely or more highly expressed by tumor and not normal tissue is a powerful tool in the study of cell regulation and growth. To this end we constructed a complementary DNA (cDNA) library from messenger RNA (mRNA) isolated from a human insulinoma and, by differential hybridization with cDNA from both normal pancreas and insulinoma, isolated clones more highly expressed by insulinoma. Methods. Total RNA was isolated from human insulinoma and normal pancreas and purified to mRNA by oligo (dT) column. An insulinoma cDNA library was constructed and screened with 32P-labeled cDNA from pancreas and insulinoma. Northern blots from insulinoma, pancreas, carcinomas, normal endocrine tissues, and endocrine tumors were then probed with the 32P- labeled inserts. Results. Two clones that consistently hybridized with 32P cDNA from insulinoma and not pancreas proved to represent mRNAs for insulin and the α subunit of the G(s) protein. There was a markedly higher expression (30-fold) of the gene for G(s)α in mRNA from insulinoma compared with normal pancreas by Northern blot analysis. We found G(s)α to be more highly expressed by a pheochromocytoma, a corticotropin-producing islet cell tumor of the pancreas, and a corticotropin-producing thymic carcinoid (up to 35-fold) compared with normal pancreas, whereas normal endocrine tissues, a parathyroid adenoma, thyroid follicular adenoma, gastrinoma, and several carcinomas showed no expression. Conclusions. This study showed that G(s)α is highly expressed in insulinoma and certain endocrine tumors. It is not expressed in several cancers or normal endocrine tissues. Others have implicated mutated G(s) proteins in the tumorigenesis of pituitary and thyroid tumors. G proteins are also known to mediate hormonal transmembrane signaling. Its overexpression in four of seven endocrine tumors tested suggests that it may have a role in the unregulated hormone secretion and/or a role in the tumorigenesis of differentiated endocrine tumors.",
author = "Zeiger, {M. A.} and Norton, {J. A.} and Harken, {A. H.} and M. Demeure",
year = "1993",
language = "English (US)",
volume = "114",
pages = "458--463",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - G(s)alpha-identification of a gene highly expressed by insulinoma and other endocrine tumors

AU - Zeiger, M. A.

AU - Norton, J. A.

AU - Harken, A. H.

AU - Demeure, M.

PY - 1993

Y1 - 1993

N2 - Background. The isolation of mRNA molecules that are either uniquely or more highly expressed by tumor and not normal tissue is a powerful tool in the study of cell regulation and growth. To this end we constructed a complementary DNA (cDNA) library from messenger RNA (mRNA) isolated from a human insulinoma and, by differential hybridization with cDNA from both normal pancreas and insulinoma, isolated clones more highly expressed by insulinoma. Methods. Total RNA was isolated from human insulinoma and normal pancreas and purified to mRNA by oligo (dT) column. An insulinoma cDNA library was constructed and screened with 32P-labeled cDNA from pancreas and insulinoma. Northern blots from insulinoma, pancreas, carcinomas, normal endocrine tissues, and endocrine tumors were then probed with the 32P- labeled inserts. Results. Two clones that consistently hybridized with 32P cDNA from insulinoma and not pancreas proved to represent mRNAs for insulin and the α subunit of the G(s) protein. There was a markedly higher expression (30-fold) of the gene for G(s)α in mRNA from insulinoma compared with normal pancreas by Northern blot analysis. We found G(s)α to be more highly expressed by a pheochromocytoma, a corticotropin-producing islet cell tumor of the pancreas, and a corticotropin-producing thymic carcinoid (up to 35-fold) compared with normal pancreas, whereas normal endocrine tissues, a parathyroid adenoma, thyroid follicular adenoma, gastrinoma, and several carcinomas showed no expression. Conclusions. This study showed that G(s)α is highly expressed in insulinoma and certain endocrine tumors. It is not expressed in several cancers or normal endocrine tissues. Others have implicated mutated G(s) proteins in the tumorigenesis of pituitary and thyroid tumors. G proteins are also known to mediate hormonal transmembrane signaling. Its overexpression in four of seven endocrine tumors tested suggests that it may have a role in the unregulated hormone secretion and/or a role in the tumorigenesis of differentiated endocrine tumors.

AB - Background. The isolation of mRNA molecules that are either uniquely or more highly expressed by tumor and not normal tissue is a powerful tool in the study of cell regulation and growth. To this end we constructed a complementary DNA (cDNA) library from messenger RNA (mRNA) isolated from a human insulinoma and, by differential hybridization with cDNA from both normal pancreas and insulinoma, isolated clones more highly expressed by insulinoma. Methods. Total RNA was isolated from human insulinoma and normal pancreas and purified to mRNA by oligo (dT) column. An insulinoma cDNA library was constructed and screened with 32P-labeled cDNA from pancreas and insulinoma. Northern blots from insulinoma, pancreas, carcinomas, normal endocrine tissues, and endocrine tumors were then probed with the 32P- labeled inserts. Results. Two clones that consistently hybridized with 32P cDNA from insulinoma and not pancreas proved to represent mRNAs for insulin and the α subunit of the G(s) protein. There was a markedly higher expression (30-fold) of the gene for G(s)α in mRNA from insulinoma compared with normal pancreas by Northern blot analysis. We found G(s)α to be more highly expressed by a pheochromocytoma, a corticotropin-producing islet cell tumor of the pancreas, and a corticotropin-producing thymic carcinoid (up to 35-fold) compared with normal pancreas, whereas normal endocrine tissues, a parathyroid adenoma, thyroid follicular adenoma, gastrinoma, and several carcinomas showed no expression. Conclusions. This study showed that G(s)α is highly expressed in insulinoma and certain endocrine tumors. It is not expressed in several cancers or normal endocrine tissues. Others have implicated mutated G(s) proteins in the tumorigenesis of pituitary and thyroid tumors. G proteins are also known to mediate hormonal transmembrane signaling. Its overexpression in four of seven endocrine tumors tested suggests that it may have a role in the unregulated hormone secretion and/or a role in the tumorigenesis of differentiated endocrine tumors.

UR - http://www.scopus.com/inward/record.url?scp=0027181092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027181092&partnerID=8YFLogxK

M3 - Article

C2 - 8342148

AN - SCOPUS:0027181092

VL - 114

SP - 458

EP - 463

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -