Growth regulation of cultured human nevus cells

Maria Laura Mancianti, Tibor Györfi, Ie Ming Shih, Istvan Valyi-Nagy, Gwendolin Levengood, Hans D. Menssen, Allan C. Halpern, David E. Elder, Meenhard Herlyn

Research output: Contribution to journalArticle

Abstract

Cells isolated from congenital melanocytic nevi and cultured in vitro have growth characteristics that resemble their premalignant stage in situ. A serum-free, chemically defined medium has been developed that allows continuous growth of established nevus cultures for up to several months. Like primary melanoma cells, nevus cells in high-calciumcontaining W489 medium require insulin for growth. In contrast to melanoma cells, nevus cells in serum-free medium require the presence of α-melanocyte-stimulating hormone, which enhanced intracellular levels of cyclic adenosine monophosphate. In contrast to the requirements of normal human melanocytes from newborn foreskin, congenital nevus cells grow with less dependency on basic fibroblast growth factor (bFGF). Nevus cultures contain bFGF-like activity, and they express bFGF mRNA. Nevic cells of compound nevi also express bFGF mRNA in situ but only in the junctional areas. These results indicate that bFGF plays an important growth regulatory role for nevus cells in vitro and in vivo.

Original languageEnglish (US)
JournalJournal of Investigative Dermatology
Volume100
Issue number3 SUPPL.
StatePublished - Mar 1993
Externally publishedYes

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Nevus
Fibroblast Growth Factor 2
Growth
Nevi and Melanomas
Melanocyte-Stimulating Hormones
Messenger RNA
Serum-Free Culture Media
Cyclic AMP
Foreskin
Pigmented Nevus
Melanocytes
Insulin
Serum

ASJC Scopus subject areas

  • Dermatology

Cite this

Mancianti, M. L., Györfi, T., Shih, I. M., Valyi-Nagy, I., Levengood, G., Menssen, H. D., ... Herlyn, M. (1993). Growth regulation of cultured human nevus cells. Journal of Investigative Dermatology, 100(3 SUPPL.).

Growth regulation of cultured human nevus cells. / Mancianti, Maria Laura; Györfi, Tibor; Shih, Ie Ming; Valyi-Nagy, Istvan; Levengood, Gwendolin; Menssen, Hans D.; Halpern, Allan C.; Elder, David E.; Herlyn, Meenhard.

In: Journal of Investigative Dermatology, Vol. 100, No. 3 SUPPL., 03.1993.

Research output: Contribution to journalArticle

Mancianti, ML, Györfi, T, Shih, IM, Valyi-Nagy, I, Levengood, G, Menssen, HD, Halpern, AC, Elder, DE & Herlyn, M 1993, 'Growth regulation of cultured human nevus cells', Journal of Investigative Dermatology, vol. 100, no. 3 SUPPL..
Mancianti ML, Györfi T, Shih IM, Valyi-Nagy I, Levengood G, Menssen HD et al. Growth regulation of cultured human nevus cells. Journal of Investigative Dermatology. 1993 Mar;100(3 SUPPL.).
Mancianti, Maria Laura ; Györfi, Tibor ; Shih, Ie Ming ; Valyi-Nagy, Istvan ; Levengood, Gwendolin ; Menssen, Hans D. ; Halpern, Allan C. ; Elder, David E. ; Herlyn, Meenhard. / Growth regulation of cultured human nevus cells. In: Journal of Investigative Dermatology. 1993 ; Vol. 100, No. 3 SUPPL.
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