Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates

P. E. Pizao, D. M. Lyaruu, G. J. Peters, J. Van Ark-Otte, B. Winograd, G. Giaccone, H. M. Pinedo

Research output: Contribution to journalArticle

Abstract

This study assessed the growth pattern, cellular organisation and chemosensitivity of established human tumour cell lines growing as postconfluent cultures in 'V'-bottomed, 96-well microtiter plates. Cross-sections of the colon (HT29, SW620, SW1116), ovarian (A2780) and head and neck (UM-SCC-22B) carcinoma microcultures allowed in situ evaluation of the cellular organisation in the wells. After 5 days of growth, every cell line had reached confluence, but each of them displayed a specific pattern of cell stacking which ranged from two to ten layers. Postconfluent HT29 cells displayed morphologic features suggestive of some degree of enterocytic differentiation. Growth and cytotoxicity could be studied reliably and reproducibly in this system with the sulforhodamine B protein assay. Against HT29 postconfluent cultures, the EC50's (drug concentrations producing absorbance readings 50% lower than those of non-treated wells) of 5-fluorouracil and of the ether lipid, hexadecylphosphocholine, were 1 mM and 50 μM respectively. The possibility to perform chemosensitivity tests using semiautomated microtiter plate technology supports further evaluation of this system as an alternative antitumour drug testing model.

Original languageEnglish (US)
Pages (from-to)660-665
Number of pages6
JournalBritish Journal of Cancer
Volume66
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Cultured Cells
miltefosine
lissamine rhodamine B
Growth
HT29 Cells
Tumor Cell Line
Fluorouracil
Antineoplastic Agents
Ether
Reading
Colon
Neck
Head
Technology
Carcinoma
Lipids
Cell Line
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pizao, P. E., Lyaruu, D. M., Peters, G. J., Van Ark-Otte, J., Winograd, B., Giaccone, G., & Pinedo, H. M. (1992). Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates. British Journal of Cancer, 66(4), 660-665.

Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates. / Pizao, P. E.; Lyaruu, D. M.; Peters, G. J.; Van Ark-Otte, J.; Winograd, B.; Giaccone, G.; Pinedo, H. M.

In: British Journal of Cancer, Vol. 66, No. 4, 1992, p. 660-665.

Research output: Contribution to journalArticle

Pizao, PE, Lyaruu, DM, Peters, GJ, Van Ark-Otte, J, Winograd, B, Giaccone, G & Pinedo, HM 1992, 'Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates', British Journal of Cancer, vol. 66, no. 4, pp. 660-665.
Pizao PE, Lyaruu DM, Peters GJ, Van Ark-Otte J, Winograd B, Giaccone G et al. Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates. British Journal of Cancer. 1992;66(4):660-665.
Pizao, P. E. ; Lyaruu, D. M. ; Peters, G. J. ; Van Ark-Otte, J. ; Winograd, B. ; Giaccone, G. ; Pinedo, H. M. / Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates. In: British Journal of Cancer. 1992 ; Vol. 66, No. 4. pp. 660-665.
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