TY - JOUR
T1 - Growth inhibitory protein(s) in the 3T3 cell plasma membrane. Partial purification and dissociation of growth inhibitory events from inhibition of amino acid transport
AU - Raben, Dan
AU - Lieberman, Michael A.
AU - Glaser, Luis
PY - 1981/7
Y1 - 1981/7
N2 - It has been reported previously that a plasma membrane‐enriched fraction from 3T3 cells arrests the growth of sparse 3T3 cells early in the G1 phase of growth (Whittenberger et al., ′78, ′79). Addition of membranes to sparse 3T3 cells also results in a decrease of the rate of transport of αaminoisobutyric acid (Lieberman et al., ′79). We have partially purified the growth‐inhibitory proteins from plasma membrane of 3T3 cells. These growth‐inhibitory proteins behave as intrinsic membrane proteins and do not require membrane lipids for activity. The partially purified proteins arrest cell growth without affecting the rate of α‐aminoisobutyric transport; thus, inhibition of both transport and cell growth are not obligatorily coupled events.
AB - It has been reported previously that a plasma membrane‐enriched fraction from 3T3 cells arrests the growth of sparse 3T3 cells early in the G1 phase of growth (Whittenberger et al., ′78, ′79). Addition of membranes to sparse 3T3 cells also results in a decrease of the rate of transport of αaminoisobutyric acid (Lieberman et al., ′79). We have partially purified the growth‐inhibitory proteins from plasma membrane of 3T3 cells. These growth‐inhibitory proteins behave as intrinsic membrane proteins and do not require membrane lipids for activity. The partially purified proteins arrest cell growth without affecting the rate of α‐aminoisobutyric transport; thus, inhibition of both transport and cell growth are not obligatorily coupled events.
UR - http://www.scopus.com/inward/record.url?scp=0019490740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019490740&partnerID=8YFLogxK
U2 - 10.1002/jcp.1041080106
DO - 10.1002/jcp.1041080106
M3 - Article
C2 - 7263766
AN - SCOPUS:0019490740
VL - 108
SP - 35
EP - 45
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 1
ER -