Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

Albert Levva, Gilberto Schwartsmann, Leonie C M Boeije, Herbert M. Pinedo, Frans de Waal

Research output: Contribution to journalArticlepeer-review

Abstract

In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.

Original languageEnglish (US)
Pages (from-to)629-635
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume141
Issue number2
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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