Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

Albert Levva; Gilberto Schwartsmann; Leonie C M Boeije; Herbert M. Pinedo; Frans de Waal

doi:10.1016/S0006-291X(86)80219-4

Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

Albert Levva, Gilberto Schwartsmann, Leonie C M Boeije, Herbert M. Pinedo, Frans de Waal

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.

Original language	English (US)
Pages (from-to)	629-635
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	141
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80219-4
State	Published - Dec 15 1986
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction",

abstract = "In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.",

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T1 - Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

AU - Levva, Albert

AU - Schwartsmann, Gilberto

AU - Boeije, Leonie C M

AU - Pinedo, Herbert M.

AU - de Waal, Frans

PY - 1986/12/15

Y1 - 1986/12/15

N2 - In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.

AB - In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.

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Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

Abstract

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