Growth hormone receptors in the porcine testis during prepuberty

M. R. N'Diaye, S. S. Sun, S. P. Fanua, K. J. Loseth, E. F. Shaw Wilgis, B. G. Crabo

Research output: Contribution to journalArticle

Abstract

Supplementation of exogenous growth hormone (GH) during prepuberty advances onset of spermatogenesis in boars, but the mechanism of action is unknown. The present study is an investigation of the presence and characteristics of testicular growth hormone receptors (GHR). A total of 36 boars were castrated, three boars every 10 days, between the ages of 10 and 120 days. Testicular membrane preparations of 10, 20, 30, 50, 70, 100 and 120-day-old boars were used to determine 125I-bGH binding and Scatchard analysis. Liver from a 60-kg barrow was used for comparison. Specific 125I-bGH binding to testicular membrane preparations occurred in all age groups with the exception of 20-day-old boars at levels of 30-40% of liver binding. At 30 days of age the unlabelled bGH at 1.1 ng/tube achieved half maximal inhibition (ID50). Results of Scatchard analysis indicated a single class of binding sites. Binding affinity was 2.89 x 109 M with a binding capacity of 12 fmole/mg membrane protein. The results from this study suggest that GH may act directly on the cells of the prepubertal boar testis.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalReproduction in Domestic Animals
Volume37
Issue number5
DOIs
StatePublished - Oct 2002

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Endocrinology

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    N'Diaye, M. R., Sun, S. S., Fanua, S. P., Loseth, K. J., Shaw Wilgis, E. F., & Crabo, B. G. (2002). Growth hormone receptors in the porcine testis during prepuberty. Reproduction in Domestic Animals, 37(5), 305-309. https://doi.org/10.1046/j.1439-0531.2002.00364.x