Growth hormone and proopiomelanocortin are targeted by autoantibodies in a patient with biopsy-proven IgG4-related hypophysitis

M. A. Landek-Salgado, P. Leporati, I. Lupi, Abby Geis, Patrizio P Caturegli

Research output: Contribution to journalArticle

Abstract

Hypophysitis is a chronic inflammation of the pituitary gland often caused by autoimmunity. Among the autoimmune diseases it is one of the few where the autoantigens remain to be identified. The goal of the paper was to characterize the antigenic profile in a previously reported patient with IgG4-related hypophysitis. Immunofluorescence and immunoblotting were performed to detect antibodies to human pituitary proteins. The proteins recognized by western blotting were then submitted to mass spectrometry for sequencing. The patient's autoantibodies recognized two unique bands around 40 and 30 kDa on immunoblotting. Sequencing revealed one peptide from proopiomelanocortin in the 40 kDa band and four peptides from growth hormone in the 30 kDa band. This work represents the first antigenic profile in IgG4-related hypophysitis, and the first recognition of proopiomelanocortin as a possible pituitary autoantigen. In addition, the work supports previous suggestions of growth hormone as a pituitary autoantigen. Further studies are needed to prove the pathogenicity and diagnostic utility of these two pituitary proteins.

Original languageEnglish (US)
Pages (from-to)412-419
Number of pages8
JournalPituitary
Volume15
Issue number3
DOIs
StatePublished - Sep 2012

Fingerprint

Pro-Opiomelanocortin
Autoantibodies
Growth Hormone
Autoantigens
Biopsy
Immunoblotting
Proteins
Peptide Hormones
Pituitary Gland
Autoimmunity
Autoimmune Diseases
Fluorescent Antibody Technique
Virulence
Mass Spectrometry
Western Blotting
Inflammation
Peptides
Antibodies
Autoimmune Hypophysitis

Keywords

  • Growth hormone
  • Hypophysitis
  • Pituitary antibodies
  • Pituitary autoantigens
  • Proopiomelanocortin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Growth hormone and proopiomelanocortin are targeted by autoantibodies in a patient with biopsy-proven IgG4-related hypophysitis. / Landek-Salgado, M. A.; Leporati, P.; Lupi, I.; Geis, Abby; Caturegli, Patrizio P.

In: Pituitary, Vol. 15, No. 3, 09.2012, p. 412-419.

Research output: Contribution to journalArticle

Landek-Salgado, M. A. ; Leporati, P. ; Lupi, I. ; Geis, Abby ; Caturegli, Patrizio P. / Growth hormone and proopiomelanocortin are targeted by autoantibodies in a patient with biopsy-proven IgG4-related hypophysitis. In: Pituitary. 2012 ; Vol. 15, No. 3. pp. 412-419.
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