TY - JOUR
T1 - Growth and integration of neocartilage with native cartilage in vitro
AU - Zhang, Zijun
AU - McCaffery, J. Michael
AU - Spencer, Richard G.S.
AU - Francomano, Clair A.
N1 - Funding Information:
This study was funded by the NIA Intramural Research Program, National Institutes of Health. The antibodies, SP1-D8, 9/30/8-A-4 and II-II6B3, were obtained from the Developmental Studies Hybridoma Bank, The University of Iowa, IA.
PY - 2005/3
Y1 - 2005/3
N2 - Poor integration of neocartilage with recipient has been a major obstacle to articular cartilage restoration. An in vitro study was designed to provide insights regarding the integration process. Cartilage explants and chondrocytes were harvested from the distal sternum of 16-day-old chick embryos. Four million chondrocytes and one 1 mm3 explant were centrifuged together in a 0.75 ml tube. In the constructs, consisting of cartilage explant and chondrocyte pellet, isolated chondrocytes attached to the surface of the explant at the beginning of the culture, followed by significant chondrocyte death at the interface between chondrocyte pellet and explant. Chondrocyte apoptosis was seen almost exclusively at this interface. Meanwhile, the interface was a zone with active extracellular matrix deposition as demonstrated by immunohistochemistry. By two weeks, the junction of neocartilage and native cartilage explant had formed an acellular zone with collagen fibrils orientated parallel with the surface of the cartilage explant. In conclusion, chondrocyte death leads to acellularity and fibril network reorganization at the neocartilage/ explant interface, and impacts the quality of cartilage repair as abnormal matrix remodeling implies.
AB - Poor integration of neocartilage with recipient has been a major obstacle to articular cartilage restoration. An in vitro study was designed to provide insights regarding the integration process. Cartilage explants and chondrocytes were harvested from the distal sternum of 16-day-old chick embryos. Four million chondrocytes and one 1 mm3 explant were centrifuged together in a 0.75 ml tube. In the constructs, consisting of cartilage explant and chondrocyte pellet, isolated chondrocytes attached to the surface of the explant at the beginning of the culture, followed by significant chondrocyte death at the interface between chondrocyte pellet and explant. Chondrocyte apoptosis was seen almost exclusively at this interface. Meanwhile, the interface was a zone with active extracellular matrix deposition as demonstrated by immunohistochemistry. By two weeks, the junction of neocartilage and native cartilage explant had formed an acellular zone with collagen fibrils orientated parallel with the surface of the cartilage explant. In conclusion, chondrocyte death leads to acellularity and fibril network reorganization at the neocartilage/ explant interface, and impacts the quality of cartilage repair as abnormal matrix remodeling implies.
KW - Apoptosis
KW - Cartilage
KW - Chondrocytes
KW - Integration
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U2 - 10.1016/j.orthres.2004.08.028
DO - 10.1016/j.orthres.2004.08.028
M3 - Article
C2 - 15734259
AN - SCOPUS:14244263463
SN - 0736-0266
VL - 23
SP - 433
EP - 439
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 2
ER -