Grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. Diagnostic implications

A. Von Graevenitz, G. Osterhout, J. Dick

Research output: Contribution to journalArticle

Abstract

One hundred and ninety-four strains of aerobically growing Gram-positive rods of the genera Corynebacterium, Actinomyces, Arcanobacterium, Erysipelothrix, Listeria, Oerskovia, Nocardia, Rhodococcus, and of unnamed Center for Disease Control (CDC) groups were checked for cellular fatty acid profiles with the Microbial Identification System (Microbial ID, Newark, Del., USA). In order to obtain unified data usable for the clinical laboratory, 24 or 48 h sheep blood agar cultures were used. It was thought that grouping and perhaps identification bould be aided by this approach. With the aid of numerical analysis, four groups (two consisting of two subgroups each) were estblished. The discriminatory ability of the scheme, however, was only 77.2%, indicating that grouping of an unknown isolate could be done with some accuracy, but that speciation would still require biochemical testing.

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalAPMIS
Volume99
Issue number2
StatePublished - Jan 1 1991

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Keywords

  • Fatty acids
  • Gram-positive rods

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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