TY - JOUR
T1 - Green tea modulation of inducible nitric oxide synthase in hypoxic/reoxygenated cardiomyocytes
AU - Agnetti, G.
AU - Bordoni, A.
AU - Angeloni, C.
AU - Leoncini, E.
AU - Guarnieri, C.
AU - Caldarera, C. M.
AU - Biagi, P. L.
AU - Hrelia, S.
N1 - Funding Information:
This work was supported by grants from MIUR, Rome, Compagnia di San Paolo, Turin and Fondazione del Monte di Bologna e Ravenna (Italy).
PY - 2005/5
Y1 - 2005/5
N2 - Hypoxia/reoxygenation (H/R) is one of the causes of the increased expression of inducible nitric oxide synthase (iNOS) in cardiomyocytes. Since an aberrant NOS induction has detrimental consequences, we evaluated the effect of a green tea extract (GTE) on the NOS induction and activity in H/R-cardiomyocytes to define a nutritional strategy. Cultured rat cardiomyocytes were exposed to H/R in the presence of two concentrations of a green tea extract (GTE), which is reported to inhibit NOS expression and activity in different cells. In cultured cardiomyocytes two NOS isoforms were constitutively expressed, but only iNOS was induced by H/R. GTE supplementation at the lowest concentration, comparable to that in human plasma after dietary consumption, was ineffective, while the highest, comparable to that achievable by dietary supplements, counteracted the effect of H/R on iNOS induction and activity. It is necessary to verify in humans the relationship between the modulation of NO production and green tea dietary consumption.
AB - Hypoxia/reoxygenation (H/R) is one of the causes of the increased expression of inducible nitric oxide synthase (iNOS) in cardiomyocytes. Since an aberrant NOS induction has detrimental consequences, we evaluated the effect of a green tea extract (GTE) on the NOS induction and activity in H/R-cardiomyocytes to define a nutritional strategy. Cultured rat cardiomyocytes were exposed to H/R in the presence of two concentrations of a green tea extract (GTE), which is reported to inhibit NOS expression and activity in different cells. In cultured cardiomyocytes two NOS isoforms were constitutively expressed, but only iNOS was induced by H/R. GTE supplementation at the lowest concentration, comparable to that in human plasma after dietary consumption, was ineffective, while the highest, comparable to that achievable by dietary supplements, counteracted the effect of H/R on iNOS induction and activity. It is necessary to verify in humans the relationship between the modulation of NO production and green tea dietary consumption.
KW - Cardiomyocytes
KW - Catechins
KW - Green tea
KW - Hypoxia/reoxygenation
KW - Nitric oxide synthase
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U2 - 10.1016/j.biochi.2005.01.004
DO - 10.1016/j.biochi.2005.01.004
M3 - Article
C2 - 15820752
AN - SCOPUS:16844363316
SN - 0300-9084
VL - 87
SP - 457
EP - 460
JO - Biochimie
JF - Biochimie
IS - 5
ER -