Green Fluorescent Protein Bone Marrow Cells Express Hematopoietic and Neural Antigens in Culture and Migrate Within the Neonatal Rat Brain

Jennifer E. Hudson, N. Chen, S. Song, P. Walczak, P. Jendelová, E. Sykova, A. E. Willing, S. Saporta, P. Bickford, J. Sanchez-Ramos, T. Zigova

Research output: Contribution to journalArticle

Abstract

Finding a reliable source of alternative neural stem cells for treatment of various diseases and injuries affecting the central nervous system is a challenge. Numerous studies have shown that hematopoietic and nonhematopoietic progenitors derived from bone marrow (BM) under specific conditions are able to differentiate into cells of all three germ layers. Recently, it was reported that cultured, unfractionated (whole) adult BM cells form nestin-positive spheres that can later initiate neural differentiation (Kabos et al., 2002). The identity of the subpopulation of BM cells that contributes to neural differentiation remains unknown. We therefore analyzed the hematopoietic and neural features of cultured, unfractionated BM cells derived from a transgenic mouse that expresses green fluorescent protein (GFP) in all tissues. We also transplanted the BM cells into the subventricular zone (SVZ), a region known to support postnatal neurogenesis. After injection of BM cells into the neurogenic SVZ in neonatal rats, we found surviving GFP+ BM cells close to the injection site and in various brain regions, including corpus callosum and subcortical white matter. Many of the grafted cells were detected within the rostral migratory stream (RMS), moving toward the olfactory bulb (OB), and some cells reached the subependymal zone of the OB. Our in vitro experiments revealed that murine GFP+ BM cells retained their proliferation and differentiation potential and predominantly preserved their hematopoietic identity (CD45, CD90, CD133), although a few expressed neural antigens (nestin, glial fibrillary acdiic protein, TuJ1).

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalJournal of neuroscience research
Volume76
Issue number2
DOIs
StatePublished - Apr 15 2004
Externally publishedYes

Keywords

  • Bone marrow
  • Grafting
  • Green mouse
  • Subventricular zone, developing rat brain

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Green Fluorescent Protein Bone Marrow Cells Express Hematopoietic and Neural Antigens in Culture and Migrate Within the Neonatal Rat Brain'. Together they form a unique fingerprint.

  • Cite this

    Hudson, J. E., Chen, N., Song, S., Walczak, P., Jendelová, P., Sykova, E., Willing, A. E., Saporta, S., Bickford, P., Sanchez-Ramos, J., & Zigova, T. (2004). Green Fluorescent Protein Bone Marrow Cells Express Hematopoietic and Neural Antigens in Culture and Migrate Within the Neonatal Rat Brain. Journal of neuroscience research, 76(2), 255-264. https://doi.org/10.1002/jnr.20043