Gradient-enhanced 3D NOESY-HMQC spectroscopy

Geerten W. Vuister; Rolf Boelens; Robert Kaptein; Maurits Burgering; Peter C.M. van Zijl

doi:10.1007/BF01875323

Gradient-enhanced 3D NOESY-HMQC spectroscopy

Geerten W. Vuister, Rolf Boelens, Robert Kaptein, Maurits Burgering, Peter C.M. van Zijl

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to¹⁵N-labeled Mnt repressor (1-76) in¹H₂O. Both coherence selection and¹H₂O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules.

Original language	English (US)
Pages (from-to)	301-305
Number of pages	5
Journal	Journal of Biomolecular NMR
Volume	2
Issue number	3
DOIs	https://doi.org/10.1007/BF01875323
State	Published - May 1992
Externally published	Yes

Keywords

3D NMR
Gradient-enhanced NMR
Mnt repressor
NOESY-HMQC

ASJC Scopus subject areas

Biochemistry
Spectroscopy

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10.1007/BF01875323

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title = "Gradient-enhanced 3D NOESY-HMQC spectroscopy",

abstract = "A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to15N-labeled Mnt repressor (1-76) in1H2O. Both coherence selection and1H2O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules.",

keywords = "3D NMR, Gradient-enhanced NMR, Mnt repressor, NOESY-HMQC",

author = "Vuister, {Geerten W.} and Rolf Boelens and Robert Kaptein and Maurits Burgering and {van Zijl}, {Peter C.M.}",

year = "1992",

month = may,

doi = "10.1007/BF01875323",

language = "English (US)",

volume = "2",

pages = "301--305",

journal = "Journal of Biomolecular NMR",

issn = "0925-2738",

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T1 - Gradient-enhanced 3D NOESY-HMQC spectroscopy

AU - Vuister, Geerten W.

AU - Boelens, Rolf

AU - Kaptein, Robert

AU - Burgering, Maurits

AU - van Zijl, Peter C.M.

PY - 1992/5

Y1 - 1992/5

N2 - A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to15N-labeled Mnt repressor (1-76) in1H2O. Both coherence selection and1H2O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules.

AB - A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to15N-labeled Mnt repressor (1-76) in1H2O. Both coherence selection and1H2O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules.

KW - 3D NMR

KW - Gradient-enhanced NMR

KW - Mnt repressor

KW - NOESY-HMQC

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JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 3

ER -

Gradient-enhanced 3D NOESY-HMQC spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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