Gp120 activates children's brain endothelial cells via CD4

Monique Stins, Y. Shen, S. H. Huang, F. Gilles, V. K. Kalra, Kwang Sik Kim

Research output: Contribution to journalArticle

Abstract

Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.

Original languageEnglish (US)
Pages (from-to)125-134
Number of pages10
JournalJournal of NeuroVirology
Volume7
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Endothelial Cells
Brain
HIV-1
Monocytes
AIDS Dementia Complex
Vascular Cell Adhesion Molecule-1
Brain Diseases
Intercellular Adhesion Molecule-1
Microvessels
HIV Infections
Anti-Idiotypic Antibodies
Interleukin-6
Observation

Keywords

  • CD4
  • gp120
  • HIV-1
  • Human cerebral microvessel endothelium
  • ICAM-1
  • Transendothelial migration of monocytes
  • VCAM-1

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

Cite this

Gp120 activates children's brain endothelial cells via CD4. / Stins, Monique; Shen, Y.; Huang, S. H.; Gilles, F.; Kalra, V. K.; Kim, Kwang Sik.

In: Journal of NeuroVirology, Vol. 7, No. 2, 2001, p. 125-134.

Research output: Contribution to journalArticle

Stins, Monique ; Shen, Y. ; Huang, S. H. ; Gilles, F. ; Kalra, V. K. ; Kim, Kwang Sik. / Gp120 activates children's brain endothelial cells via CD4. In: Journal of NeuroVirology. 2001 ; Vol. 7, No. 2. pp. 125-134.
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