Gp120 activates children's brain endothelial cells via CD4

M. F. Stins; Y. Shen; S. H. Huang; F. Gilles; V. K. Kalra; K. S. Kim

doi:10.1080/13550280152058780

Gp120 activates children's brain endothelial cells via CD4

M. F. Stins, Y. Shen, S. H. Huang, F. Gilles, V. K. Kalra, K. S. Kim

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.

Original language	English (US)
Pages (from-to)	125-134
Number of pages	10
Journal	Journal of neurovirology
Volume	7
Issue number	2
DOIs	https://doi.org/10.1080/13550280152058780
State	Published - 2001
Externally published	Yes

Keywords

CD4
HIV-1
Human cerebral microvessel endothelium
ICAM-1
Transendothelial migration of monocytes
VCAM-1
gp120

ASJC Scopus subject areas

Neurology
Clinical Neurology
Cellular and Molecular Neuroscience
Virology

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10.1080/13550280152058780

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title = "Gp120 activates children's brain endothelial cells via CD4",

abstract = "Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.",

keywords = "CD4, HIV-1, Human cerebral microvessel endothelium, ICAM-1, Transendothelial migration of monocytes, VCAM-1, gp120",

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T1 - Gp120 activates children's brain endothelial cells via CD4

AU - Stins, M. F.

AU - Shen, Y.

AU - Huang, S. H.

AU - Gilles, F.

AU - Kalra, V. K.

AU - Kim, K. S.

PY - 2001

Y1 - 2001

N2 - Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.

AB - Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.

KW - CD4

KW - HIV-1

KW - Human cerebral microvessel endothelium

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KW - Transendothelial migration of monocytes

KW - VCAM-1

KW - gp120

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Gp120 activates children's brain endothelial cells via CD4

Abstract

Keywords

ASJC Scopus subject areas

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