Abstract
Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-134 |
| Number of pages | 10 |
| Journal | Journal of NeuroVirology |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
Fingerprint
Keywords
- CD4
- gp120
- HIV-1
- Human cerebral microvessel endothelium
- ICAM-1
- Transendothelial migration of monocytes
- VCAM-1
ASJC Scopus subject areas
- Virology
- Clinical Neurology
Cite this
Gp120 activates children's brain endothelial cells via CD4. / Stins, Monique; Shen, Y.; Huang, S. H.; Gilles, F.; Kalra, V. K.; Kim, Kwang Sik.
In: Journal of NeuroVirology, Vol. 7, No. 2, 2001, p. 125-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gp120 activates children's brain endothelial cells via CD4
AU - Stins, Monique
AU - Shen, Y.
AU - Huang, S. H.
AU - Gilles, F.
AU - Kalra, V. K.
AU - Kim, Kwang Sik
PY - 2001
Y1 - 2001
N2 - Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.
AB - Encephalopathy represents a common and serious manifestation of HIV-1 infection in children, but its pathogenesis is unclear. We demonstrated that gp120 activated human brain microvascular endothelial cells (HBMEC) derived from children in up-regulating ICAM-1 and VCAM-1 expression, IL-6 secretion and increased monocyte transmigration across monolayers. Another novel observation was our demonstration of CD4 in isolated HBMEC and on microvessels of children's brain cryosections. Gp120-induced monocyte migration was inhibited by anti-gp120 and anti-CD4 antibodies. This is the first demonstration that gp120 activates HBMEC via CD4, which may contribute to the development of HIV-1 encephalopathy in children.
KW - CD4
KW - gp120
KW - HIV-1
KW - Human cerebral microvessel endothelium
KW - ICAM-1
KW - Transendothelial migration of monocytes
KW - VCAM-1
UR - http://www.scopus.com/inward/record.url?scp=0034916179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034916179&partnerID=8YFLogxK
U2 - 10.1080/13550280152058780
DO - 10.1080/13550280152058780
M3 - Article
C2 - 11517385
AN - SCOPUS:0034916179
VL - 7
SP - 125
EP - 134
JO - Journal of NeuroVirology
JF - Journal of NeuroVirology
SN - 1355-0284
IS - 2
ER -