Goofy coordinates the acuity of olfactory signaling

Tomomi Kaneko-Goto, Yuki Sato, Sayako Katada, Emi Kinameri, Sei Ichi Yoshihara, Atsushi Nishiyori, Mitsuhiro Kimura, Hiroko Fujita, Kazushige Touhara, Randall R Reed, Yoshihiro Yoshihara

Research output: Contribution to journalArticle

Abstract

The basic scheme of odor perception and signaling from olfactory cilia to the brain is well understood. However, factors that affect olfactory acuity of an animal, the threshold sensitivity to odorants, are less well studied. Using signal sequence trap screening of a mouse olfactory epithelium cDNA library, we identified a novel molecule, Goofy, that is essential for olfactory acuity in mice. Goofy encodes an integral membrane protein with specific expression in the olfactory and vomeronasal sensory neurons and predominant localization to the Golgi compartment. Goofy-deficient mice display aberrant olfactory phenotypes, including the impaired trafficking of adenylyl cyclase III, stunted olfactory cilia, and a higher threshold for physiological and behavioral responses to odorants. In addition, the expression of dominant-negative form of cAMP-dependent protein kinase results in shortening of olfactory cilia, implying a possible mechanistic link between cAMP and ciliogenesis in the olfactory sensory neurons. These results demonstrate that Goofy plays an important role in establishing the acuity of olfactory sensory signaling.

Original languageEnglish (US)
Pages (from-to)12987-12996
Number of pages10
JournalJournal of Neuroscience
Volume33
Issue number32
DOIs
StatePublished - 2013

Fingerprint

Cilia
Olfactory Receptor Neurons
Olfactory Perception
Olfactory Mucosa
Protein Sorting Signals
Cyclic AMP-Dependent Protein Kinases
Gene Library
Membrane Proteins
Phenotype
Brain
Odorants

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kaneko-Goto, T., Sato, Y., Katada, S., Kinameri, E., Yoshihara, S. I., Nishiyori, A., ... Yoshihara, Y. (2013). Goofy coordinates the acuity of olfactory signaling. Journal of Neuroscience, 33(32), 12987-12996. https://doi.org/10.1523/JNEUROSCI.4948-12.2013

Goofy coordinates the acuity of olfactory signaling. / Kaneko-Goto, Tomomi; Sato, Yuki; Katada, Sayako; Kinameri, Emi; Yoshihara, Sei Ichi; Nishiyori, Atsushi; Kimura, Mitsuhiro; Fujita, Hiroko; Touhara, Kazushige; Reed, Randall R; Yoshihara, Yoshihiro.

In: Journal of Neuroscience, Vol. 33, No. 32, 2013, p. 12987-12996.

Research output: Contribution to journalArticle

Kaneko-Goto, T, Sato, Y, Katada, S, Kinameri, E, Yoshihara, SI, Nishiyori, A, Kimura, M, Fujita, H, Touhara, K, Reed, RR & Yoshihara, Y 2013, 'Goofy coordinates the acuity of olfactory signaling', Journal of Neuroscience, vol. 33, no. 32, pp. 12987-12996. https://doi.org/10.1523/JNEUROSCI.4948-12.2013
Kaneko-Goto T, Sato Y, Katada S, Kinameri E, Yoshihara SI, Nishiyori A et al. Goofy coordinates the acuity of olfactory signaling. Journal of Neuroscience. 2013;33(32):12987-12996. https://doi.org/10.1523/JNEUROSCI.4948-12.2013
Kaneko-Goto, Tomomi ; Sato, Yuki ; Katada, Sayako ; Kinameri, Emi ; Yoshihara, Sei Ichi ; Nishiyori, Atsushi ; Kimura, Mitsuhiro ; Fujita, Hiroko ; Touhara, Kazushige ; Reed, Randall R ; Yoshihara, Yoshihiro. / Goofy coordinates the acuity of olfactory signaling. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 32. pp. 12987-12996.
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