GOLPH2 and MYO6

Putative prostate cancer markers localized to the golgi apparatus

Shuanzeng Wei, Thomas A. Dunn, William B Isaacs, Angelo Michael Demarzo, Jun Luo

Research output: Contribution to journalArticle

Abstract

BACKGROUND. Malignant transformation is often accompanied by morphological and functional alterations in subcellular organelles. The Golgi apparatus is a subcellular structure primarily involved in modification and sorting of macromolecules for secretion and transport to other cellular destinations. Molecular alterations associated with the Golgi apparatus may take place during prostate carcinogenesis but such alterations have not been documented. METHODS. To demonstrate that the Golgi apparatus undergoes alterations during prostate carcinogenesis, we examined the expression and localization of two candidate molecules, Golgi phosphoprotein 2 (GOLPH2) and myosin VI (MYO6), both overexpressed in prostate cancer as initially identified by expression microarray analysis. RESULTS. Elevated GOLPH2 expression in prostate cancers was validated through real-time RT-PCR, Western blot, and tissue microarray analysis, and its Golgi localization in surgical prostate cancer tissues confirmed using two-color immunofluorescence. In addition, distinctive juxtanuclear MYO6 staining pattern consistent with Golgi localization was observed in surgical prostate cancer tissues. Two-color immunofluorescence revealed intensive Golgi-specific staining for both GOLPH2 and myosin VI in prostate cancer cells but not in the adjacent normal prostate epithelium. CONCLUSIONS. We show that the Golgi apparatus in prostate cancer cells differs from the normal Golgi by elevated levels of two molecules, GOLPH2 and MYO6. These results for the first time demonstrated consistent cancer cell-specific alterations in the molecular composition of the Golgi apparatus. Such alterations can be explored for discovery of novel prostate cancer biomarkers through targeted organellar approaches.

Original languageEnglish (US)
Pages (from-to)1387-1395
Number of pages9
JournalProstate
Volume68
Issue number13
DOIs
StatePublished - Sep 15 2008

Fingerprint

Phosphoproteins
Golgi Apparatus
Prostatic Neoplasms
Prostate
Fluorescent Antibody Technique
Carcinogenesis
Color
Tissue Array Analysis
Staining and Labeling
Microarray Analysis
Tumor Biomarkers
Organelles
Real-Time Polymerase Chain Reaction
Epithelium
Western Blotting
Neoplasms

Keywords

  • Golgi
  • GOLPH2
  • Myosin VI
  • Prostate cancer

ASJC Scopus subject areas

  • Urology
  • Medicine(all)

Cite this

GOLPH2 and MYO6 : Putative prostate cancer markers localized to the golgi apparatus. / Wei, Shuanzeng; Dunn, Thomas A.; Isaacs, William B; Demarzo, Angelo Michael; Luo, Jun.

In: Prostate, Vol. 68, No. 13, 15.09.2008, p. 1387-1395.

Research output: Contribution to journalArticle

@article{3a7d6e9f571c48e8939e4a45560731f5,
title = "GOLPH2 and MYO6: Putative prostate cancer markers localized to the golgi apparatus",
abstract = "BACKGROUND. Malignant transformation is often accompanied by morphological and functional alterations in subcellular organelles. The Golgi apparatus is a subcellular structure primarily involved in modification and sorting of macromolecules for secretion and transport to other cellular destinations. Molecular alterations associated with the Golgi apparatus may take place during prostate carcinogenesis but such alterations have not been documented. METHODS. To demonstrate that the Golgi apparatus undergoes alterations during prostate carcinogenesis, we examined the expression and localization of two candidate molecules, Golgi phosphoprotein 2 (GOLPH2) and myosin VI (MYO6), both overexpressed in prostate cancer as initially identified by expression microarray analysis. RESULTS. Elevated GOLPH2 expression in prostate cancers was validated through real-time RT-PCR, Western blot, and tissue microarray analysis, and its Golgi localization in surgical prostate cancer tissues confirmed using two-color immunofluorescence. In addition, distinctive juxtanuclear MYO6 staining pattern consistent with Golgi localization was observed in surgical prostate cancer tissues. Two-color immunofluorescence revealed intensive Golgi-specific staining for both GOLPH2 and myosin VI in prostate cancer cells but not in the adjacent normal prostate epithelium. CONCLUSIONS. We show that the Golgi apparatus in prostate cancer cells differs from the normal Golgi by elevated levels of two molecules, GOLPH2 and MYO6. These results for the first time demonstrated consistent cancer cell-specific alterations in the molecular composition of the Golgi apparatus. Such alterations can be explored for discovery of novel prostate cancer biomarkers through targeted organellar approaches.",
keywords = "Golgi, GOLPH2, Myosin VI, Prostate cancer",
author = "Shuanzeng Wei and Dunn, {Thomas A.} and Isaacs, {William B} and Demarzo, {Angelo Michael} and Jun Luo",
year = "2008",
month = "9",
day = "15",
doi = "10.1002/pros.20806",
language = "English (US)",
volume = "68",
pages = "1387--1395",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "13",

}

TY - JOUR

T1 - GOLPH2 and MYO6

T2 - Putative prostate cancer markers localized to the golgi apparatus

AU - Wei, Shuanzeng

AU - Dunn, Thomas A.

AU - Isaacs, William B

AU - Demarzo, Angelo Michael

AU - Luo, Jun

PY - 2008/9/15

Y1 - 2008/9/15

N2 - BACKGROUND. Malignant transformation is often accompanied by morphological and functional alterations in subcellular organelles. The Golgi apparatus is a subcellular structure primarily involved in modification and sorting of macromolecules for secretion and transport to other cellular destinations. Molecular alterations associated with the Golgi apparatus may take place during prostate carcinogenesis but such alterations have not been documented. METHODS. To demonstrate that the Golgi apparatus undergoes alterations during prostate carcinogenesis, we examined the expression and localization of two candidate molecules, Golgi phosphoprotein 2 (GOLPH2) and myosin VI (MYO6), both overexpressed in prostate cancer as initially identified by expression microarray analysis. RESULTS. Elevated GOLPH2 expression in prostate cancers was validated through real-time RT-PCR, Western blot, and tissue microarray analysis, and its Golgi localization in surgical prostate cancer tissues confirmed using two-color immunofluorescence. In addition, distinctive juxtanuclear MYO6 staining pattern consistent with Golgi localization was observed in surgical prostate cancer tissues. Two-color immunofluorescence revealed intensive Golgi-specific staining for both GOLPH2 and myosin VI in prostate cancer cells but not in the adjacent normal prostate epithelium. CONCLUSIONS. We show that the Golgi apparatus in prostate cancer cells differs from the normal Golgi by elevated levels of two molecules, GOLPH2 and MYO6. These results for the first time demonstrated consistent cancer cell-specific alterations in the molecular composition of the Golgi apparatus. Such alterations can be explored for discovery of novel prostate cancer biomarkers through targeted organellar approaches.

AB - BACKGROUND. Malignant transformation is often accompanied by morphological and functional alterations in subcellular organelles. The Golgi apparatus is a subcellular structure primarily involved in modification and sorting of macromolecules for secretion and transport to other cellular destinations. Molecular alterations associated with the Golgi apparatus may take place during prostate carcinogenesis but such alterations have not been documented. METHODS. To demonstrate that the Golgi apparatus undergoes alterations during prostate carcinogenesis, we examined the expression and localization of two candidate molecules, Golgi phosphoprotein 2 (GOLPH2) and myosin VI (MYO6), both overexpressed in prostate cancer as initially identified by expression microarray analysis. RESULTS. Elevated GOLPH2 expression in prostate cancers was validated through real-time RT-PCR, Western blot, and tissue microarray analysis, and its Golgi localization in surgical prostate cancer tissues confirmed using two-color immunofluorescence. In addition, distinctive juxtanuclear MYO6 staining pattern consistent with Golgi localization was observed in surgical prostate cancer tissues. Two-color immunofluorescence revealed intensive Golgi-specific staining for both GOLPH2 and myosin VI in prostate cancer cells but not in the adjacent normal prostate epithelium. CONCLUSIONS. We show that the Golgi apparatus in prostate cancer cells differs from the normal Golgi by elevated levels of two molecules, GOLPH2 and MYO6. These results for the first time demonstrated consistent cancer cell-specific alterations in the molecular composition of the Golgi apparatus. Such alterations can be explored for discovery of novel prostate cancer biomarkers through targeted organellar approaches.

KW - Golgi

KW - GOLPH2

KW - Myosin VI

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=50949101502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50949101502&partnerID=8YFLogxK

U2 - 10.1002/pros.20806

DO - 10.1002/pros.20806

M3 - Article

VL - 68

SP - 1387

EP - 1395

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 13

ER -